Aspartic Acid – Reference Materials for Accurate Quantification
Aspartic acid — High-Purity Reference Materials for CONFIDENT Quantification
Accelerate your amino acid analytics with HPC Standards’ Aspartic acid reference materials and 13C/15N‑labelled derivatives. Achieve traceable calibration, robust method validation, and consistent QC across LC‑MS/MS, HPLC/UPLC, GC‑MS (derivatized), and CE workflows. Supplied with comprehensive CoA, SI traceability, and SDS, our neat and solution RMs deliver batch-to-batch reliability for food, pharma, biomedical, and environmental labs. Custom mixes, matrix-matched standards, and technical support available to fit your method and regulatory needs.
Product | Catalog No./ CAS No. | Quantity | Price | |
|---|---|---|---|---|
ISO 17034 Reference Material |  | 691096 | 1X100MG | |
ISO 17034 Reference Material |  | 690766 | 1X100MG | Please log in. |
ISO 17034 Certified Reference Material L-Aspartic acid solution |  | 694011 | 1X5ML | Please log in. |
HPC Standards provides high-purity aspartic acid reference materials and stable isotope–labelled derivatives for reliable calibration, method validation, and quality control in food, pharmaceutical, biomedical, and environmental laboratories.
Overview
Aspartic acid (L-aspartic acid; CAS 56-84-8) is a proteinogenic, acidic amino acid with side-chain carboxyl functionality. Formula: C4H7NO4; molecular weight: 133.10 g/mol. Key acid-base properties: pKa1 ≈ 2.1 (α-COOH), pKa2 ≈ 3.9 (side-chain COOH), pKa3 ≈ 9.8 (α-NH3+); isoelectric point ≈ 2.8. It appears as a white crystalline solid, is sparingly soluble in water, and forms more soluble salts (e.g., sodium/potassium aspartate). Reference materials enable traceable quantification of free aspartic acid and total aspartate after hydrolysis.
Uses
- Calibration and system suitability for amino acid profiling by LC-MS/MS, HPLC (pre/post-column derivatization), ion-exchange chromatography, and capillary electrophoresis.
- Internal standardization (using 13C/15N-labelled aspartic acid) in targeted metabolomics, proteomics, and clinical chemistry.
- Quality control of protein hydrolysates, fermentation broths, dietary supplements, parenteral nutrition mixtures, and cell culture media.
- Food authenticity testing, protein digestibility studies, stability studies of biologics, and bioprocess monitoring.
Regulatory & Compliance
- Chemicals management: subject to EU REACH and CLP; aspartic acid is generally not classified as hazardous under GHS when pure and uncontaminated (verify current SDS).
- Applicable quality frameworks for analytical use include ISO/IEC 17034 (reference material production), ISO/IEC 17025 (testing), and ISO 9001 (quality management).
- Method validation should follow ICH Q2(R2)/Q14, ISO 17025 guidance, and sector-specific SOPs (food, pharma, clinical).
Monitoring
- Matrices: plasma/serum, urine, cerebrospinal fluid, tissues, cell lysates, bioreactor media, protein hydrolysates, food ingredients, and wastewater/effluents.
- Typical workflows: protein precipitation or SPE, derivatization (e.g., OPA/FMOC, AQC, PITC) for HPLC/CE; direct or mild derivatization for LC-MS/MS; GC-MS requires suitable silylation/esterification.
- Use matrix-matched calibration and isotopically labelled internal standards to correct for recovery and ion suppression.
Health Impact — Human Toxicity
- Aspartic acid is a natural dietary amino acid with low acute toxicity at typical exposure levels. It is widely present in proteins and metabolic pathways.
- Occupational exposure: dust may cause mechanical irritation to eyes, skin, and respiratory tract. No known chronic systemic toxicity at occupational levels when handled properly.
- Follow SDS for handling; avoid inhalation of powders and contact with eyes. Not intended for therapeutic use.
Environmental Impact — Effects on Wildlife
- Aspartic acid is readily biodegradable and exhibits low bioaccumulation potential. Aquatic toxicity is expected to be low relative to many industrial chemicals.
- As a naturally occurring amino acid, environmental persistence is minimal under normal conditions; standard wastewater treatment effectively reduces concentrations.
Safety Measures
- Personal protection: lab coat, safety glasses, and suitable gloves; use dust control and local exhaust ventilation when weighing powders.
- Handling: minimize dust generation, use antistatic measures, and work in clean, dry conditions.
- First aid: rinse eyes/skin with water upon contact; move to fresh air if inhaled; consult SDS for detailed guidance.
Analytical Methods
- LC-MS/MS: preferred for sensitivity and selectivity; use 13C/15N-aspartic acid as internal standard; aqueous or mild derivatization workflows.
- HPLC/UPLC: ion-exchange with ninhydrin detection; reverse-phase with pre-column derivatization (AQC, OPA/FMOC, PITC); UV/FLD detection.
- GC-MS: requires derivatization (e.g., TBDMS, MTBSTFA, or ethyl chloroformate protocols) for volatility.
- CE: background electrolyte optimization with derivatization enhances UV/FLD detectability.
Method Performance & Validation
- Establish linearity across expected ranges (e.g., low ng/mL to high μg/mL depending on matrix).
- Assess matrix effects, recovery, precision, trueness, LOD/LOQ, and robustness per ICH Q2(R2)/ISO 17025.
- Use certified reference materials and isotopically labelled internal standards to demonstrate traceability and measurement uncertainty.
Analytical Reference Materials from HPC Standards
- Neat materials: L-aspartic acid crystalline reference materials, assay and purity characterized with full documentation.
- Solution reference materials: gravimetrically prepared concentrations in water or water/acetonitrile, with stated uncertainty and stability data.
- Isotope-labelled options: 13C- and/or 15N-labelled L-aspartic acid for internal standardization in LC-MS/MS.
- Custom matrices and mixes: tailored amino acid panels, matrix-matched RMs (e.g., serum, culture media), and custom concentrations on request.
Certification & Documentation
- Each lot supplied with Certificate of Analysis (CoA) detailing purity, identity (NMR/IR/MS), water/volatiles, residual solvents (if applicable), and uncertainty.
- Traceability to SI where applicable; gravimetric preparation with calibrated balances and verified solvent purity.
- Accompanied by Safety Data Sheet (SDS) and recommended storage/handling instructions.
Storage & Stability
- Neat material: store tightly closed in a dry, cool place (2–8 °C), protected from moisture and light.
- Solutions: store refrigerated (2–8 °C) or frozen (≤ −20 °C) depending on matrix; avoid repeated freeze–thaw cycles; note assigned shelf-life on label/CoA.
- Allow to equilibrate to room temperature before opening to prevent moisture ingress; record opening date for inventory control.
Quality & Accreditation
- Manufactured under stringent quality management aligned with international standards for reference materials.
- Batch-to-batch consistency, homogeneity testing, and stability monitoring ensure reliable performance across shelf-life.
- Suitable for audits and regulatory submissions requiring documented measurement traceability.
Applications in Food & Environmental Analysis
- Nutritional profiling of foods and feeds (free and total amino acids); verification of fortification and formulation.
- Bioprocess control (e.g., amino acid consumption/production in fermentation); wastewater monitoring for nitrogenous metabolites.
- Support for proteomic workflows and peptide mapping via hydrolysis controls and calibration.
Packaging & Ordering
- Common pack sizes: neat materials from milligram to multi-gram; solutions from 1 mL ampoules/vials to custom volumes.
- Tamper-evident, inert packaging with lot-specific labels and barcodes for LIMS integration.
- Custom concentrations, blends, and isotope enrichments available upon request to match your method and instrument needs.
Instrument Compatibility
- Validated for LC-MS/MS, HPLC/UPLC (UV/FLD), GC-MS (after derivatization), and CE methods.
- Low-adsorption packaging and solvent systems selected to minimize carryover and adsorption on tubing and vials.
Support & Services
- Technical consultation for method setup, calibration design, and uncertainty budgets.
- Custom development of matrix-matched and multi-analyte reference materials for high-throughput laboratories.
- Stability and requalification services for extended studies and long-term projects.