Methyl-5-nitroaniline Reference Materials

Methyl-5-nitroaniline Reference Materials PRECISE. RELIABLE. COMPLIANT.

Secure consistent, defensible results with high-purity Methyl-5-nitroaniline reference materials from HPC Standards. Engineered for accurate identification, quantification, and ISO-compliant method validation in environmental and industrial residue analysis, our standards are produced under rigorous quality control and delivered with complete documentation CoA, uncertainty, stability, storage. Choose from neat materials or ready-to-use LC-grade solutions in acetonitrile or methanol, with custom concentrations, multi-analyte mixes, and isotope-labelled options available. Optimize calibration, system suitability, and ongoing QC to meet regulatory requirements across REACHTSCA-driven workflowsbacked by fast lead times and expert technical support.

Product		Catalog No./ CAS No.	Quantity	Price
2-Methyl-5-nitroaniline		676173 99-55-8	1X250MG	Please log in.
2-Methyl-5-nitroaniline solution Concentration: 10.0 µg/ml Solvent: Acetonitrile 10 µg/ml Acetonitrile		681721 99-55-8	1X10ML	Please log in.

High-quality reference materials for Methyl-5-nitroaniline to support precise identification, quantification, and method validation in environmental and industrial residue analysis. Manufactured under rigorous quality control and accompanied by comprehensive documentation for regulatory compliance.

Overview

Methyl-5-nitroaniline is an aromatic amine bearing a nitro substituent and a methyl group on the aniline ring. It is typically encountered as a yellow to orange crystalline solid with low water solubility and good solubility in common organic solvents. As a nitroaniline derivative, it represents a relevant analyte in environmental and industrial monitoring due to its potential persistence and toxicity.

Key chemical features include the presence of both electron-donating (amino, methyl) and electron-withdrawing (nitro) groups, influencing its acid-base behavior, chromatographic retention, and spectroscopic characteristics.

Chemical Identity and Synonyms

Methyl-5-nitroaniline refers to a methyl-substituted nitroaniline isomer with an aniline core. Synonym usage in the literature may vary by numbering conventions; users should confirm exact isomer designation and identifiers (e.g., CAS RN) in their internal documentation prior to method setup.

Functional classes: aromatic amine, nitroaromatic compound, dye/intermediate-like substance. Typical appearance: yellow/orange crystalline solid; odorless or faint aromatic odor.

Applications and Sources

- Intermediate in organic synthesis (e.g., dyes, pigments, specialty chemicals).
- Potential impurity or transformation product in industrial processes involving nitroanilines or toluidines.
- May occur in industrial effluents, production sites, contaminated soils/sediments, and wastewater streams.

Its occurrence in environmental matrices is primarily linked to manufacturing, processing, and disposal activities.

Regulatory Status

Methyl-5-nitroaniline, as a nitroaromatic amine, is generally subject to chemical management frameworks such as REACH (EU) and TSCA (US) and to worker protection regulations addressing hazardous substances. Sector-specific requirements (e.g., emissions, wastewater discharge, waste handling) may apply depending on jurisdiction.

Users should consult current regional legislation, safety data, and permit conditions for use, storage, emissions, and disposal. Analytical data traceability is often required for regulatory reporting and audits.

Monitoring and Analytical Considerations

- Target matrices: industrial wastewater, surface/ground water, sediments, soils, indoor dust (industrial), process streams, and product quality control.
- Sample preparation: solid-phase extraction (SPE) for aqueous samples; QuEChERS or solvent extraction for solid matrices; filtration to remove particulates; consideration of pH to stabilize the amine functional group.
- Instrumentation: HPLC/UPLC with UV/Vis or DAD detection; LC-MS/MS for selectivity and sensitivity; GC-MS possible following suitable derivatization to enhance volatility and stability.

Method validation should address matrix effects, recoveries, linearity, selectivity, LOQ/LOD, and analyte stability. Reference materials are essential for calibration, quality control, and proficiency testing.

Analytical Performance and Stability

Methyl-5-nitroaniline can undergo photolysis and redox transformations; protect from light and strong oxidizing/reducing conditions. Evaluate solution stability in selected solvents (e.g., acetonitrile, methanol) and at storage temperatures recommended for the reference materials. Include stability-indicating checks within QC charts.

Health Impact

Human toxicity: Aromatic nitroanilines may cause methemoglobinemia, central nervous system effects, and irritation upon acute exposure. Chronic exposure to related aromatic amines has been associated with hematological and hepatic effects; some aromatic amines are potential carcinogens. Dermal absorption and inhalation of dust or aerosols are relevant exposure routes in occupational settings.

Implement medical surveillance where required, and ensure rapid response capability for suspected methemoglobinemia cases.

Environmental Impact

Methyl-5-nitroaniline, as a nitroaromatic compound, may exhibit persistence and toxicity in aquatic environments. Biodegradation can be limited under certain conditions, with potential formation of reduced intermediates (e.g., amino derivatives). Adsorption to sediments/soils may occur, influencing mobility and bioavailability.

Effects on Wildlife

Aquatic organisms may be sensitive to nitroaromatic amines, with potential sublethal effects on growth and reproduction. Risk assessments should consider both parent compound and transformation products, especially under reductive environmental conditions.

Occupational Exposure and Controls

- Engineering controls: closed handling, local exhaust ventilation, dust containment.
- Administrative controls: SOPs for weighing, solution preparation, spill response, and waste handling; training on hazards and PPE use.
- Personal protective equipment: chemical-resistant gloves, lab coat, eye/face protection; respiratory protection where airborne exposure cannot be adequately controlled.

Safety Measures and Handling

Store in tightly closed containers, protected from light and incompatible reagents (strong oxidizers/reductants). Maintain cool, dry conditions. Avoid skin contact, ingestion, and inhalation of dust. Use dedicated, labeled containers for standard solutions. Follow site-specific emergency procedures for spills and exposures.

Transport and Storage

Classify and ship according to applicable transport regulations for hazardous chemicals. Use secondary containment and temperature/light protection as indicated in the product documentation. On receipt, verify integrity and store according to the certificate of analysis (CoA) recommendations.

Quality, Traceability, and Documentation

Each reference material is delivered with a CoA detailing identity confirmation, purity assessment, assigned value with uncertainty, solvent system, and recommended storage. Production follows stringent quality control to support traceability and reproducibility across batches. Batch-specific stability data are provided where applicable.

HPC Standards Reference Materials

HPC Standards GmbH supplies Methyl-5-nitroaniline reference materials tailored for quantitative analysis and method validation. Offerings include neat materials and ready-to-use solutions at defined concentrations in high-purity solvents (e.g., acetonitrile or methanol), optimized for LC-based workflows.

Products are designed for laboratories conducting environmental and industrial residue analysis, supporting calibration, system suitability, and ongoing quality control to ensure compliance with regulatory limits and internal specifications.

Customization and Related Products

- Custom concentrations and solvent matrices on request.
- Mixes with structurally related aromatic amines to streamline multi-analyte methods.
- Metabolites or transformation products (where available) to support comprehensive monitoring.
- Stable isotope-labelled derivatives can be offered on request to enhance quantitation via isotope-dilution LC-MS/MS.

Method Support and Compliance

Technical data packages support ISO-compliant method validation (selectivity, linearity, trueness, precision, LOQ/LOD, robustness). Reference materials facilitate internal QC (control charts, recovery checks) and external proficiency testing performance.

Typical Use Cases

- Monitoring of industrial wastewater and effluents for nitroaniline derivatives.
- Verification of removal efficiency in treatment processes.
- Forensic and incident investigations of aromatic amine releases.
- Quality control of intermediates and finished products where carryover or impurity profiling is required.

Ordering and Availability

Multiple pack sizes and concentrations are available to match throughput and stability needs. Lead times are minimized through in-house production. Contact our technical team for selection guidance, matrix compatibility, and documentation requirements for audits.