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Hydroxytriclabendazole
Hydroxytriclabendazole Precision Reference Materials for Confident Compliance
Elevate your residue analysis with HPC Standards high-purity Hydroxytriclabendazole reference materials. Designed for LCMSMS workflows in food and environmental matrices, our certified standards enable traceable calibration, robust method validation, and reproducible quantification alongside triclabendazole, sulfoxide, and sulfone metabolites. Delivered with comprehensive CoA, purity and uncertainty data, and available with isotope-labeled internal standards on requestideal for ISOIEC 17025 and SANTE-aligned laboratories seeking reliable results at or below MRLs.
Product | Catalog No./ CAS No. | Quantity | Price | |
|---|---|---|---|---|
 | 692966 | 1X5MG | ||
D3-Hydroxytriclabendazole solution |  | 692967 | 1X1ML | |
 | 679364 | 1X10MG | Please log in. | |
Hydroxytriclabendazole solution |  | 689340 | 1X1ML | Please log in. |
Hydroxytriclabendazole solution |  | 689341 | 1X1ML | Please log in. |
Reference materials for precise determination of Hydroxytriclabendazole in food and environmental matrices, enabling compliant, reproducible residue analysis.
Overview
Hydroxytriclabendazole is a hydroxylated metabolite of the flukicide triclabendazole. It occurs in animals and environmental samples following veterinary use of triclabendazole and contributes to the overall residue definition in regulatory monitoring. Due to structural similarity to the parent compound, sensitive analytical methods are required to separate and quantify this metabolite alongside other transformation products.
In residue control, Hydroxytriclabendazole is typically assessed together with triclabendazole and key metabolites (e.g., sulfoxide and sulfone derivatives), with results often expressed as triclabendazole equivalents per regulatory definitions.
Chemical and Metabolic Context
Hydroxytriclabendazole results from phase I biotransformation (hydroxylation) of triclabendazole. It may co-occur with sulfoxidation and further oxidation products. The metabolite can be present as free, conjugated (e.g., glucuronides/sulfates), or bound residues depending on species, tissue, and time post-treatment.
Analytical workflows should consider potential conjugate hydrolysis or targeted quantification of both free and conjugated forms as stipulated in the relevant method or regulatory guidance.
Uses (Context: Parent Drug Triclabendazole)
Triclabendazole is a benzimidazole anthelmintic used in veterinary medicine against liver flukes (Fasciola spp.) in ruminants and other livestock. Hydroxytriclabendazole is not a formulated product but a diagnostic residue marker and analytical target arising from the use of triclabendazole.
Regulatory Status and MRLs
Many jurisdictions establish maximum residue limits (MRLs) for triclabendazole residues in edible tissues and milk of food-producing animals. The residue definition frequently includes triclabendazole and specified metabolites, which can include hydroxylated species, and may be expressed as triclabendazole.
Laboratories should consult the current regional regulations (e.g., EU, Codex, national veterinary drug residue frameworks) for the applicable residue definition, target matrices, MRL values, and confirmation criteria.
Residue Monitoring and Target Matrices
Typical matrices: muscle, liver, kidney, fat, milk, and sometimes plasma/serum for pharmacokinetic or withdrawal studies. Environmental monitoring may include manure, slurry, surface waters, sediments, or soils in areas with intensive livestock treatment.
Time-to-slaughter and milk withdrawal periods influence metabolite profiles; Hydroxytriclabendazole may be more prominent at certain post-treatment intervals. Method validation should cover the full residue definition and decision limits at or below the MRL/Action Level.
Sample Preparation
Common approaches include protein precipitation and liquid–liquid extraction or solid-phase extraction (SPE). For complex matrices (e.g., liver, milk), cleanup strategies such as SPE mixed-mode sorbents or dispersive techniques can improve selectivity and robustness.
Enzymatic or acidic hydrolysis may be applied when the residue definition requires determination of conjugated forms. Recovery of Hydroxytriclabendazole must be validated across concentration levels bracketing the MRL.
Analytical Methods
LC–MS/MS is the preferred technique for selective quantification at low µg/kg levels. Method development should ensure baseline separation or MRM selectivity among triclabendazole, hydroxy-, sulfoxide-, and sulfone metabolites. Isocratic or gradient reversed-phase separations with aqueous buffers and organic modifiers are common.
Key validation parameters: selectivity, matrix effects, linearity with matrix-matched calibration, accuracy (recovery), precision (intra-/inter-day), decision limit (CCα), detection capability (CCβ), carryover, stability (bench-top, freeze–thaw, long-term), and confirmation criteria (ion ratios/retention time).
Calibration and Quality Assurance
Use certified reference materials for Hydroxytriclabendazole to establish traceable calibration. Matrix-matched or standard-addition calibration mitigates ion suppression/enhancement.
Include system suitability checks, continuing calibration verification, spiked quality controls at multiple levels, and blanks to monitor contamination. Participation in proficiency tests and use of isotopically labeled internal standards improve accuracy and comparability across laboratories.
Health Impact (Human Toxicity)
Toxicological assessments for regulatory purposes are primarily based on the parent drug triclabendazole and its major metabolites. Hydroxytriclabendazole is considered within the overall residue definition used to set safe exposure limits for consumers. Adherence to MRLs ensures that consumer exposure remains below health-based guidance values established by competent authorities.
Environmental Impact
Following veterinary use, residues and metabolites may reach manure, soils, and surface waters. Hydroxylated metabolites can exhibit different persistence and mobility compared to the parent compound. Environmental risk assessments focus on predicted environmental concentrations and effects on non-target organisms.
Effects on Wildlife
Potential exposure pathways include runoff to aquatic systems and application of contaminated manure to fields. Sensitive taxa may vary; surveillance emphasizes minimizing environmental load through good veterinary and agricultural practices.
Safety Measures and Handling
Handle analytical solutions of Hydroxytriclabendazole using standard laboratory hygiene and PPE (gloves, lab coat, eye protection). Avoid inhalation, ingestion, and skin contact. Work in well-ventilated areas or fume hoods when preparing stock solutions. Dispose of waste according to local regulations.
Stability and Storage
Store reference materials in tightly closed amber vials under recommended temperature conditions to protect from light and moisture. Prepare fresh working solutions as needed and document freeze–thaw cycles. Verify stability during method validation and routine use.
Applications for Laboratories
Suitable for regulatory residue control in food safety labs, veterinary drug monitoring, pharmacokinetic studies, environmental fate assessments, and method development/validation studies requiring target quantification of triclabendazole metabolites.
Related Compounds
Frequently co-analyzed: triclabendazole (parent), triclabendazole sulfoxide, triclabendazole sulfone, and other hydroxylated metabolites. Method selectivity should address potential interferences among these analytes.
Compliance and Accreditation
Reference methods and validation should align with international guidelines (e.g., EU SANTE/ISO/IEC 17025, Codex, national residue programs). Documentation of traceability, uncertainty estimation, and quality controls supports accreditation and regulatory inspections.
Analytical Standards (Reference Materials)
HPC Standards GmbH supplies high-quality reference materials for Hydroxytriclabendazole to support accurate calibration, method validation, and routine QC in food and environmental analysis. Materials are produced under stringent quality systems and accompanied by comprehensive documentation (Certificate of Analysis, traceability, purity data).
On request, we provide related metabolites and stable isotope–labeled internal standards to enhance quantification accuracy and method robustness across diverse matrices.
Packaging and Documentation
Available in optimized package sizes for development and routine testing. Each lot is delivered with a Certificate of Analysis detailing identification, purity, uncertainty, storage recommendations, and traceability to ensure reproducible results.