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Pyridoxine hydrochloride – Reference Materials for Accurate Quantification
Pyridoxine hydrochloride Certified Reference Materials for Confident Quantification
Achieve precise, reproducible results with HPC Standards certified reference materials for pyridoxine hydrochloride vitamin B6 HCl. Purpose-built for method development, validation, and routine QC, our traceable standards support food, pharmaceutical, feed, and supplement analysisensuring label claim verification, stability studies, and regulatory compliance USPPh. Eur., ISOIEC 17025, ISO 17034 workflows. Choose from neat materials, ready-to-use solutions, or custom multi-analyte blends. Each lot ships with a comprehensive CoA and SDS, backed by expert technical support and reliable global logistics.
Product | Catalog No./ CAS No. | Quantity | Price | |
|---|---|---|---|---|
D2-Pyridoxine hydrochloride solution |  | 692625 | 1X1ML | |
 | 691285 | 1X250MG | Please log in. |
HPC Standards GmbH supplies certified reference materials for Pyridoxine hydrochloride (vitamin B6 HCl) to support precise method development, validation, and routine monitoring in food, pharmaceutical, and environmental laboratories.
Overview
Pyridoxine hydrochloride is the hydrochloride salt of pyridoxine, a form of vitamin B6. It is widely used as a vitamin supplement and as a fortificant in foods and feeds. In analytical contexts, robust quantification of pyridoxine (and other B6 vitamers) is essential for label claim verification, stability studies, and regulatory compliance.
As a water‑soluble compound, pyridoxine hydrochloride is amenable to aqueous extraction and chromatographic separation. Accurate calibration with traceable reference materials is critical to ensure comparability across laboratories and matrices.
Uses (Analytical and Industrial)
- Food and beverage: determination of vitamin B6 fortification levels in cereals, beverages, infant formula, and dietary supplements.
- Pharmaceutical QC: assay and related substances testing of finished dosage forms and APIs containing pyridoxine hydrochloride.
- Feed and premix control: verification of nutritional content in compound feeds and premixes.
- Clinical research and biomonitoring: support for method calibration in studies assessing vitamin B6 status (where permitted and applicable).
Regulatory Landscape
Pyridoxine (vitamin B6) is recognized internationally as an essential nutrient and is available as an over-the-counter or prescription product depending on jurisdiction. Food fortification levels and labeling requirements are regulated by regional authorities (e.g., EU, US). Pharmaceutical quality is governed by pharmacopeial monographs (e.g., Ph. Eur., USP) and GMP requirements for medicinal products.
Laboratories rely on validated methods and traceable reference materials to demonstrate conformity to label claims, specification limits, and shelf-life stability criteria.
Monitoring and Target Matrices
- Foods and fortified products: cereals, bars, beverages, dairy products, and infant nutrition.
- Dietary supplements: tablets, capsules, powders, and liquid preparations.
- Pharmaceutical forms: tablets, injections, and multivitamin combinations.
- Feeds and premixes: vitamin blends and complete feeds.
Health Impact – Human Toxicity
Pyridoxine (vitamin B6) is required for amino acid, carbohydrate, and lipid metabolism and supports glycogen phosphorylase activity. It is generally well tolerated at normal intake levels. Excessive exposure can cause dose-dependent adverse effects; overdose has been associated with peripheral sensory neuropathy characterized by numbness and reduced sensory perception.
Normal doses are considered safe during pregnancy and breastfeeding; pyridoxine is included on essential medicine lists in its vitamin role. Laboratories should report quantitative results with appropriate measurement uncertainty to support safe formulation and compliance with recommended intake levels.
Environmental Impact
Pyridoxine hydrochloride is a nutrient compound with low expected environmental hazard at typical use concentrations. Nonetheless, good laboratory practice recommends minimizing releases and managing waste according to local regulations.
Safety Measures (Laboratory Handling)
- Handle with standard laboratory PPE (lab coat, gloves, safety glasses).
- Avoid dust formation; use local exhaust ventilation during weighing.
- Store tightly closed in a cool, dry place, protected from light and moisture.
- Dispose of waste in accordance with institutional and local regulations.
Analytical Methods
- HPLC/UV or HPLC/FLD after derivatization for vitamers; direct UV detection for pyridoxine hydrochloride where selectivity permits.
- LC-MS/MS for specificity in complex matrices and multivitamin formulations.
- Sample preparation: aqueous or buffer extraction; consider matrix cleanup (SPE or dilution) to mitigate interferences.
- System suitability: use well-characterized reference materials for calibration curves, recovery checks, and stability-indicating methods.
Quality and Traceability
HPC Standards reference materials for pyridoxine hydrochloride are manufactured under stringent quality systems aligned with international requirements. Each lot is accompanied by a detailed certificate specifying identity, purity, concentration (for solutions), uncertainty, and traceability, supporting ISO/IEC 17025 and ISO 17034-based quality workflows.
Product Formats
- Neat reference materials with defined purity for primary calibration.
- Ready-to-use solutions in suitable solvents and concentrations for routine calibration and system suitability testing.
- Custom blends and multi-analyte mixes on request to match your method scope.
Stability and Storage
Pyridoxine derivatives can be sensitive to light and prolonged exposure to elevated temperature. Store as indicated on the certificate, typically at controlled room temperature or refrigerated conditions, protected from light and moisture. Observe shelf-life and requalification intervals.
Method Validation Support
Use HPC Standards reference materials to establish linearity, accuracy (recovery), precision, LOD/LOQ, robustness, and measurement uncertainty. Matrix-matched calibration and spike-recovery experiments are recommended for complex foods, supplements, and pharmaceutical formulations.
Applications in Compliance
- Food labeling and fortification verification according to regional regulations.
- Pharmacopoeial assay confirmation for finished dosage forms.
- Stability studies for real-time and accelerated conditions with stability-indicating LC methods.
Chemical Identity and Synonyms
Preferred name: Pyridoxine hydrochloride; common synonym: Vitamin B6 HCl. Related base form: Pyridoxine (vitamin B6). Note: The base and hydrochloride salt differ in physicochemical properties; select the correct reference material for your method and matrix.
Logistics and Documentation
Products ship with certificates of analysis and safety data sheets. Temperature-controlled shipping is available where required. Batch reservation and continuity of supply can be arranged for long-term studies.
Why HPC Standards
We are a manufacturer and distributor of reference materials with deep expertise in food and environmental analysis, pharmaceuticals, and metabolites. Our portfolio, technical support, and custom manufacturing capabilities ensure reliable results and regulatory confidence.
Analytical Standards (Reference Materials)
Explore our pyridoxine hydrochloride reference materials for calibration, QC, and proficiency testing. Contact our technical team for matrix-specific recommendations, custom concentrations, and multi-analyte solutions tailored to your method.