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Oleandomycin Phosphate
Oleandomycin phosphate Precision Reference Materials for Confident Residue Analysis
Achieve defensible results faster with HPC Standards high-purity Oleandomycin phosphate reference materials. Engineered for LCMSMS workflows, our neat and ready-to-use solutions enable accurate identification and quantification of macrolide residues across food and environmental matrices. Every lot ships with a comprehensive CoA detailing identity, purity, concentration, uncertainty, and full traceability to international quality requirementssupporting ISOIEC 17025 laboratories in calibration, validation, QC, and proficiency testing. Temperature-controlled packaging and proven stability guidance safeguard integrity from our facility to your bench. Need more Request custom concentrations, solvent systems, multi-analyte mixes, or stable isotopelabeled derivatives to minimize matrix effects and elevate quantification accuracy.
Product | Catalog No./ CAS No. | Quantity | Price | |
|---|---|---|---|---|
 | 677140 | 1X10MG | Please log in. | |
ISO 17034 Certified Reference Material Oleandomycin phosphate solution |  | 678232 | 1X1ML | Please log in. |
High-purity reference materials for oleandomycin phosphate enabling reliable identification and quantification of veterinary drug residues in food and environmental matrices, compliant with international quality requirements.
Overview
Oleandomycin phosphate is the phosphate salt of oleandomycin, a macrolide antibiotic produced by Streptomyces species. It is structurally related to erythromycin and other macrolides and is formulated as a water‑soluble salt to improve handling and dosing. In residue analysis, oleandomycin phosphate is monitored as a marker for macrolide use in food-producing animals and for environmental exposure.
Reference materials for oleandomycin phosphate support method development, calibration, quality control, and proficiency testing in accredited laboratories.
Chemical Identity and Properties
Class: macrolide antibiotic (phosphate salt). Typical attributes: moderate molecular weight macrolactone with deoxy-sugar substituents; basic functional groups; increased aqueous solubility as the phosphate salt. Sensitive to strong acids/bases, elevated temperature, and prolonged light exposure. In solution, macrolides may undergo hydrolysis or epimerization under unfavorable conditions.
Solubility: phosphate salt is readily soluble in water and polar organic solvents (e.g., methanol). Stability is enhanced in cool, dry, and dark storage with appropriate solvent selection and pH control.
Mode of Action and Class
Oleandomycin inhibits bacterial protein synthesis by binding to the 50S ribosomal subunit, blocking peptide chain elongation. It exhibits activity primarily against Gram-positive organisms and some atypical bacteria. As with other macrolides, selection pressure may promote resistance via ribosomal target modification, efflux, or enzymatic inactivation.
Uses and Applications
Historically used in veterinary medicine for treatment and metaphylaxis of bacterial infections in food-producing animals. Current use patterns vary by jurisdiction due to antimicrobial stewardship policies. Analytical monitoring focuses on residues in edible tissues and products (e.g., meat, milk, eggs) and potential environmental release.
In laboratory settings, oleandomycin phosphate reference materials are used for calibration, spiking experiments, validation studies (selectivity, recovery, precision), routine QC, and as system suitability checks in LC-based methods.
Regulatory Status
Oleandomycin residues are subject to veterinary drug regulations and maximum residue limits (MRLs) where established. Jurisdictional frameworks include EU legislation on pharmacologically active substances in food-producing animals, US FDA tolerances, and Codex Alimentarius MRLs where applicable. Many regions restrict or have phased out certain macrolide uses; laboratories should consult the latest national and regional regulations and compendia for current MRLs, target tissues, and analytical requirements.
Accredited testing commonly aligns with ISO/IEC 17025 and employs validated methods meeting performance criteria for confirmation and quantification.
Monitoring and Target Matrices
Typical food matrices: muscle, liver, kidney, fat, milk, eggs, honey, and aquaculture products. Feed and premixes may be monitored for carry-over. Environmental matrices can include manure, slurry, soil, and surface/groundwater downstream of agricultural activities. Selection of matrices is driven by regulatory scope, species, and exposure pathways.
Sample Preparation and Extraction
Common workflows use protein precipitation (e.g., acetonitrile or methanol), buffered extraction, and cleanup via SPE (e.g., reversed-phase, mixed-mode cation exchange) or matrix-matched QuEChERS adaptations for veterinary drugs. pH control is critical to maintain analyte stability and recovery for basic macrolides. Matrix effects are mitigated using internal standards, matrix-matched calibration, and appropriate cleanup.
Analytical Methods
LC–MS/MS is the primary technique for selective and sensitive determination of oleandomycin in complex matrices, enabling confirmation by multiple transitions and retention time matching. HPLC–UV/FLD may be used for screening where sensitivity allows, often with derivatization or enhanced detection strategies. Typical validation parameters include selectivity, linearity, recovery, precision, decision limit (CCα), detection capability (CCβ), and measurement uncertainty aligned with regulatory guidance.
Confirmation and Quantification
Quantification is commonly achieved via external calibration with matrix-matched standards or isotopically labeled internal standards to correct for ion suppression/enhancement. Confirmation relies on multiple MRM transitions, ion ratio tolerances, and chromatographic criteria per regulatory guidelines. Reporting limits are set relative to applicable MRLs or action levels.
Quality Assurance and Traceability
Use of traceable reference materials with documented purity, identity, and uncertainty supports defensible results. Routine QC includes system suitability, blanks, fortified matrix controls at relevant levels, and participation in proficiency tests. Documentation of lot traceability, storage conditions, and expiration ensures compliance.
Human Toxicity
Macrolides can cause gastrointestinal effects and, in sensitized individuals, allergic or respiratory reactions. Laboratory exposure may occur via inhalation of aerosols or dermal contact with solutions or powders. Standard laboratory hygiene and exposure controls minimize risk. Consumers are protected through adherence to MRLs, withdrawal times, and regulatory surveillance programs.
Environmental Impact and Effects on Wildlife
Release of macrolide antibiotics to the environment may contribute to selection for antimicrobial resistance in microbial communities. Potential sublethal effects on aquatic microorganisms and primary producers can occur at elevated concentrations. Environmental monitoring focuses on effluents, manure-amended soils, and receiving waters to assess occurrence and risk.
Residue Stability and Storage
Oleandomycin phosphate solutions are sensitive to hydrolysis and should be prepared in suitable solvents, stored cold, and protected from light. Solid reference materials should be kept tightly closed in inert containers with desiccation, at recommended temperatures to prevent degradation. Sample stability in matrices depends on temperature, pH, and storage time; frozen storage and rapid analysis are recommended.
Stability and re-test dates provided with each lot support scheduling and data defensibility.
Cross-Reactivity and Interferences
Structurally related macrolides (e.g., erythromycin, tylosin, spiramycin) may co-elute or share fragments; chromatographic resolution and specific MRM transitions mitigate interference. Matrix co-extractives (lipids, phospholipids) can suppress ionization; cleanup and isotope-dilution strategies reduce bias.
Safety Measures and Handling
Handle as a hazardous laboratory chemical: avoid inhalation and skin contact, use appropriate PPE (lab coat, gloves, eye protection), and work in a fume hood when preparing solutions. Prevent cross-contamination by using dedicated tools and clean areas for reference material handling. Dispose of waste according to local regulations for antibiotic-containing materials.
Reference Materials from HPC Standards
HPC Standards provides high-purity reference materials for oleandomycin phosphate to support qualitative and quantitative residue analysis in food and environmental testing. Each product is supplied with a comprehensive Certificate of Analysis detailing identity confirmation, purity, concentration (for solutions), uncertainty, and traceability to international quality requirements.
Our portfolio includes neat materials and ready-to-use solutions at defined concentrations for streamlined calibration and QC, optimized for LC–MS/MS workflows.
Packaging, Storage, and Shipping
Products are packaged in inert, low-adsorption containers to preserve integrity. Recommended storage is cool, dry, and protected from light; many solutions require refrigerated or frozen storage as indicated on the label and CoA. Temperature-controlled shipping options ensure material stability during transit, with tamper-evident seals and lot-specific documentation.
Related Compounds and Metabolites
Analysis may include related macrolides and transformation products depending on regulatory scope. Method development should evaluate potential in vivo or in vitro conversion between salt forms and the free base, as well as degradants formed during processing or storage.
Custom Solutions and Isotopically Labeled Derivatives
On request, HPC Standards offers custom concentrations, solvent systems, multi-analyte mixtures, and matrix-matched spiking solutions. Stable isotope-labeled derivatives and tailored internal standard solutions are available to enhance quantification accuracy and compensate for matrix effects.
Documentation and Compliance
Each lot is delivered with a Certificate of Analysis and safety documentation to support ISO/IEC 17025 and ISO 9001 quality systems. Our reference materials are manufactured and tested according to international quality requirements, supporting compliance with regional and global regulatory frameworks for veterinary drug residue control.