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Naphthylacetamide (primarily 1-Naphthaleneacetamide, 1-NAD)
Naphthylacetamide HighPurity Reference Materials for Confident Residue Analysis
Accelerate compliant, ISOready workflows with HPC Standards Naphthylacetamide 1NAD reference materials. Our highpurity neat standards and calibrated solutions enable precise LCMSMS quantification, robust method validation, and reliable QC across food and environmental matrices. Benefit from batchspecific CoAs, traceable multitechnique characterization NMRLCMS, stability guidance, and optional multianalyte mixes including 1NAA and isotopelabelled derivatives. Flexible pack sizes, custom concentrations, and expert application support help your lab meet regulatory MRLs with confidence and consistency.
Product | Catalog No./ CAS No. | Quantity | Price | |
|---|---|---|---|---|
ISO 17034 Reference Material |  | 676441 | 1X500MG | Please log in. |
ISO 17034 Certified Reference Material 1-Naphthylacetamide solution |  | 682916 | 1X5ML | Please log in. |
ISO 17034 Certified Reference Material |  | 690307 | 1X100MG | Please log in. |
HPC Standards GmbH provides high‑purity reference materials for Naphthylacetamide to support precise residue analysis in food and environmental laboratories, ensuring regulatory compliance and robust method validation.
Overview
Naphthylacetamide typically refers to 1-Naphthaleneacetamide (1-NAD), a synthetic plant growth regulator of the auxin class. It is used to promote rooting, regulate fruit set, and manage thinning in various horticultural crops. In analytical contexts, it is monitored as a residue in plant-derived foods and environmental samples due to its agricultural use and potential formation from related substances.
Multiple positional isomers exist (e.g., 1- and 2-naphthylacetamide). The 1-isomer (1-NAD) is the one commonly relevant in agrochemical applications and residue monitoring.
Synonyms and Identifiers
Common name: 1-Naphthaleneacetamide; 1-Naphthylacetamide; 1-NAD.
Related compounds: 1-Naphthaleneacetic acid (1-NAA; a metabolite/related auxin), 2-Naphthylacetamide (positional isomer).
CAS (1-NAD): 86-86-2. Molecular formula: C12H11NO. Approx. molecular weight: 185.22 g/mol.
Uses
Plant growth regulator for horticulture: promotion of adventitious roots, regulation of fruit set and thinning in orchards, ornamental propagation, and nursery applications. Often applied as foliar sprays, dips, or rooting preparations.
Research and quality control: used as an analyte and system suitability compound in the development, validation, and routine operation of residue-monitoring methods in food and environmental laboratories.
Physicochemical Properties
Structure: naphthalene ring substituted with an acetamide side chain. The amide functional group moderates polarity relative to the corresponding acid (1-NAA).
Typical behavior: low to moderate water solubility; good solubility in common organic solvents (e.g., acetonitrile, methanol). Moderately lipophilic (log P in the low-to-mid 2 range, matrix-dependent). Thermal stability suitable for LC-based methods; GC analysis usually requires derivatization.
Regulatory
Regulatory status and maximum residue limits (MRLs) for Naphthylacetamide (1-NAD) vary by jurisdiction. Authorities may define specific MRLs for plant growth regulators in fruit and vegetable commodities, with corresponding residue definitions that can include parent compound and/or metabolites (e.g., 1-NAA).
Laboratories should consult current legislation and databases (e.g., EU MRLs, US tolerances, Codex) for commodity-specific limits, residue definitions, and analytical scope.
Monitoring
Target matrices: fresh produce (pome fruits, stone fruits, berries), leafy greens, herbs, ornamental cuttings, and environmental samples (irrigation water, runoff, soils near treated areas).
Sampling considerations: representative sampling across treated lots and timepoints post-application; protect samples from light and heat; freeze or refrigerate promptly to minimize degradation and biotransformation.
Analytical Methods
Primary techniques: LC-MS/MS (preferred for sensitivity and selectivity) and HPLC-UV/FLD where applicable. GC-MS feasible following suitable derivatization.
Sample preparation: QuEChERS-based extraction and dispersive SPE clean-up for plant matrices; SPE (e.g., reversed-phase or mixed-mode) for water and more complex extracts. Method validation typically includes matrix-matched calibration and isotopically labeled internal standards where available.
Performance targets: LOQs in the low µg/kg range for food matrices depending on regulatory needs; routine QC with blanks, spikes, and recovery studies aligned to SANTE/ISO guidance.
Health Impact
Human toxicity: Naphthylacetamide (1-NAD) is generally of low acute toxicity but can cause eye and skin irritation. Ingestion or inhalation of concentrated material may be harmful. No classification as a carcinogen is widely recognized; however, users should consult current SDS data and local classifications.
Metabolism: In plants and possibly in biotic systems, 1-NAD can be converted to 1-naphthaleneacetic acid (1-NAA), an active auxin. Monitoring strategies may consider both parent and relevant metabolites where required by residue definitions.
Environmental Impact
Fate: Potential for adsorption to organic matter in soils; limited volatility. Degradation may occur via biotic pathways and hydrolysis over time, with potential formation of related naphthaleneacetic derivatives.
Effects on wildlife: Like many plant growth regulators, acute ecotoxicity is typically moderate; however, precaution is warranted for aquatic organisms. Environmental risk is context-dependent on application rates, timing, and local conditions.
Safety Measures
Handling: Use in a fume hood; wear appropriate PPE (lab coat, nitrile gloves, safety glasses). Avoid inhalation of dusts/aerosols and contact with skin or eyes. Implement good laboratory hygiene practices.
Storage: Keep tightly closed, protected from light, dry, and cool (typically 2–8 °C). For solutions, store in amber containers at low temperature; check stability data and requalification intervals.
Disposal: Treat waste as hazardous; dispose according to local regulations. Prevent release to the environment.
Quality and Compliance
HPC Standards reference materials for Naphthylacetamide are produced and tested according to international quality requirements to meet the highest industrial standards. Each lot is accompanied by documentation detailing purity, identity confirmation, and uncertainty estimation to support ISO-compliant method validation and routine QC.
Traceability: Characterization by orthogonal techniques (e.g., NMR, LC, MS); batch-specific Certificates of Analysis and stability guidance support defensible data in regulated environments.
Analytical Standards
Available formats: neat high-purity reference materials, ready-to-use calibrated solutions at custom concentrations in suitable solvents (e.g., acetonitrile), and multi-analyte mixes for screening workflows.
Support scope: parent compound (1-Naphthaleneacetamide), related analytes such as 1-Naphthaleneacetic acid (1-NAA), and, where available, stable isotope-labeled derivatives to enhance quantitation accuracy.
Method Development Guidance
Recommended LC-MS/MS: reversed-phase C18; mobile phases with aqueous buffer (e.g., 0.1% formic acid or ammonium formate) and organic modifier (acetonitrile/methanol). Monitor multiple transitions for confirmation. Include matrix-matched calibration and internal standardization.
QC design: include system suitability with reference materials, ongoing recovery checks (spikes at LOQ and 10× LOQ), and participation in proficiency testing where applicable.
Storage and Stability
Solid reference materials: store at 2–8 °C in original amber containers; protect from moisture and light. Allow to equilibrate to room temperature before opening to prevent condensation.
Solution standards: store refrigerated or frozen per CoA; track opening date and assign in-use expiry. Avoid repeated freeze–thaw cycles; prepare working dilutions fresh as needed.
Sample Matrices and Expected Interferences
Common matrices: apples, pears, citrus, berries, leafy greens, ornamental cuttings, water, and soil extracts. Pigmented or high-wax matrices may require additional clean-up to mitigate ion suppression.
Potential interferences: co-extracted plant hormones, phenolics, and matrix components; mitigate via optimized d-SPE sorbents and chromatographic separation.
Related Analytes and Metabolites
Consider inclusion of 1-NAA and other auxin-type regulators in multi-residue methods. Depending on regulatory residue definitions, hydrolysis or conjugate cleavage steps may be evaluated during method validation.
Applications in Laboratories
Food safety testing for MRL compliance; environmental monitoring near treated fields and greenhouses; R&D on plant growth regulator behavior; stability and migration studies in postharvest treatments.
Procurement and Customization
HPC Standards GmbH offers flexible pack sizes, custom concentrations, solvent systems, and multi-component mixes to align with your SOPs. Technical documentation and application support are available to streamline accreditation-ready implementation.