Niclosamide – Reference Materials for Residue Analysis

Niclosamide Precision Reference Materials for Confident Residue Analysis

Ensure regulatory-ready results with high-purity Niclosamide standards from HPC Standards. Our ISO-level tested reference materials deliver traceable identity, verified purity, and consistent performance for LC-MSMS workflows in food and environmental laboratories. Choose from neat materials, ready-to-use solutions, and custom mixesincluding stable isotopelabelled optionsto accelerate method validation, routine calibration, and proficiency testing. Supplied with comprehensive CoAs, robust stability guidance, and expert technical support for seamless compliance and audit readiness.

Product		Catalog No./ CAS No.	Quantity	Price
Niclosamide		674464 50-65-7	1X100MG	Please log in.
Niclosamide solution Concentration: 100 µg/ml Solvent: Acetonitrile		694278 50-65-7	1X1ML	Please log in.

High-purity niclosamide reference materials from HPC Standards support precise quantification in food and environmental testing, enabling regulatory compliance and method validation.

Overview

Niclosamide (CAS 50-65-7) is a salicylanilide compound used as an anthelmintic in human and veterinary medicine and as a molluscicide/lampricide in environmental control programs. It acts primarily as an uncoupler of oxidative phosphorylation. Molecular formula: C13H8Cl2N2O4; typical analysis targets include parent niclosamide in diverse matrices.

Key identifiers: EC Number 200-059-2; common synonyms include niclosamide anhydrous; UNII: YU55MQ3BIK. Analytical workflows commonly employ LC-MS/MS for selective determination at low µg/kg levels.

Uses

- Therapeutic: Oral anthelmintic for human tapeworm infections; limited systemic absorption, local action in the gastrointestinal tract.

- Veterinary/animal health: Control of cestode infections in animals (per product labels and local approvals).

- Environmental management: Registered as a molluscicide and as a lampricide (often in combination regimes) for invasive fish control in freshwater systems.

Regulatory Status

Niclosamide may fall under multiple regulatory frameworks depending on use: human/veterinary medicinal product regulations, and biocidal/pesticide regulations for molluscicidal or lampricidal applications.

Maximum residue limits (MRLs) and tolerances vary by jurisdiction and commodity. Laboratories should consult current databases (e.g., EU Pesticides Database, national MRL lists, Codex) for up-to-date limits and approved uses. Product-specific authorizations and water quality criteria may apply for environmental applications.

Monitoring and Residue Control

- Target matrices: fish and aquaculture products, drinking and surface waters, sediments, biota, animal-derived foods (where relevant), and wastewater.

- Objectives: surveillance of treatment programs, verification of compliance with MRLs or environmental quality standards, method validation, and proficiency testing.

- Analytes: parent niclosamide; depending on scope, include related salicylanilides or transformation products as method controls.

Health Impact (Human Toxicity)

Niclosamide exhibits low systemic absorption when administered orally; acute toxicity is generally low at therapeutic doses. Reported adverse effects are primarily gastrointestinal (e.g., nausea, abdominal discomfort). Severe reactions are uncommon.

Toxicological profiles indicate mitochondrial uncoupling as the mode of action; standard occupational exposure controls are indicated for concentrated material. Risk assessments for residues focus on dietary intake relative to established health-based guidance values where applicable.

Environmental Impact

Niclosamide is highly toxic to fish and aquatic invertebrates; it is intentionally used in controlled settings for target species management. Environmental fate includes photolysis and hydrolysis pathways; sorption to sediments can occur. Persistence and mobility depend on pH, light, and organic content.

Effects on wildlife: Aquatic organisms are most sensitive; avian and mammalian toxicity is comparatively lower. Non-target impacts are mitigated through controlled dosing, site-specific risk assessment, and monitoring of water concentrations and downstream recovery.

Safety Measures (Laboratory Handling)

- Handle powders and solutions in a fume hood; avoid dust/aerosol generation. Use gloves, lab coat, and appropriate eye protection.

- Store tightly closed in a cool, dry, and dark place. Protect reference materials from light to minimize degradation.

- Dispose of niclosamide-containing waste according to local hazardous waste regulations; avoid release to drains or surface waters.

Analytical Methods

- Instrumentation: LC-MS/MS with ESI (commonly negative mode) is preferred for selectivity and sensitivity; HPLC-UV can be used for higher-level determination.

- Sample preparation: Liquid–liquid extraction or SPE (e.g., reversed-phase or polymeric sorbents). For food matrices, QuEChERS variants with pH control can be adapted; for water, on-cartridge enrichment is typical.

- Performance: Method validation should cover linearity, recovery, matrix effects, precision, LOQ in the low µg/L or µg/kg range, and analyte stability (light and pH sensitivity).

Stability and Storage

Niclosamide can undergo photodegradation and pH-dependent transformation. Store solid and solution reference materials protected from light at controlled temperature. Prepare fresh working solutions periodically and document stability as part of method QA.

Matrices of Interest

- Aquatic: surface water, sediment, effluent, and biota (fish tissue).

- Food and feed: fish and aquaculture products; other commodities as required by regulatory programs.

- Pharma/veterinary: dosage forms and in-process controls for quality checks (where analytical reference is needed).

Quality and Compliance

HPC Standards provides niclosamide reference materials tested to international quality requirements and manufactured under rigorous QC to ensure identity, purity, and traceable characterization. Each item is supplied with a certificate of analysis detailing purity, uncertainty (where applicable), storage conditions, and intended use.

Reference Materials from HPC Standards

- Niclosamide neat reference material for calibration and spiking.

- Ready-to-use niclosamide solutions at defined concentrations for routine calibration and system suitability.

- Custom matrices, concentrations, and mixes on request, including inclusion in pesticide or veterinary screening mixes.

Related Compounds and Mixes

On request, HPC Standards can supply structurally related salicylanilides or method-specific multi-analyte mixes to support screening, confirmation, and QC workflows. Stable isotope-labelled derivatives may be available to improve quantification via isotope dilution.

Documentation and Support

Comprehensive CoAs, safety data sheets, and technical guidance for method development are provided. Our technical team supports selection of appropriate reference materials, matrix compatibility, and storage/stability planning for accredited workflows.

Shipping and Handling

Products are packed to preserve integrity during transit, with temperature and light protection as required. Batch traceability and tamper-evident packaging support audit readiness for regulated laboratories.