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Ethyl-2-thiouracil — Reference Materials for Residue Analysis
Ethyl-2-thiouracil Precision Reference Materials for Confident Residue Control
Secure your compliance workflows with Ethyl-2-thiouracil reference materials from HPC Standards. Our high-purity, traceable standards enable robust screening, confirmation, and quantification in complex matrices urine, tissues, feed, optimized for LCMSMS methods. Delivered with comprehensive certificates and uncertainty budgets, they support matrix-matched calibration, isotopelabelled internal standardization, and proficiency testing. Choose neat or ready-to-use solutions, custom concentrations, and panel blends with related thiouracilsbacked by expert technical support for method validation and regulatory alignment.
Product | Catalog No./ CAS No. | Quantity | Price | |
|---|---|---|---|---|
 | 693098 | 1X10MG | ||
13C,15N2-5-Ethyl-2-thiouracil solution |  | 693099 | 1X1ML | |
 | 680329 | 1X50MG | Please log in. | |
 | 680320 | 1X10MG | Please log in. |
HPC Standards provides high-quality reference materials for Ethyl-2-thiouracil to support precise residue testing in food and environmental matrices, ensuring regulatory compliance and robust method validation.
Overview
Ethyl-2-thiouracil (an ethylated derivative of 2-thiouracil) is a thiouracil-class antithyroid compound. It inhibits thyroid hormone biosynthesis by interfering with thyroid peroxidase-mediated iodination and coupling reactions. In residue control, Ethyl-2-thiouracil is monitored as a marker for the illegal use of antithyroid agents in food-producing animals and as part of confirmatory panels alongside related thiouracils.
HPC Standards reference materials enable accurate qualitative and quantitative determination of Ethyl-2-thiouracil in complex matrices, supporting screening, confirmation, and proficiency testing.
Chemical Profile
Class: Thiouracils (antithyroid agents). Structural motif: pyrimidinone with thione (C=S) functionality and an ethyl substitution on the ring nitrogen. Typical properties: polar, weakly acidic, moderate water solubility, amenable to LC–MS/MS detection.
Stability depends on solvent, pH, light, and temperature; neat materials and solutions should be protected from moisture and light.
Uses
Historically used as an antithyroid drug class; derivatives have been misused as growth-promoting agents in livestock due to metabolic effects associated with reduced thyroid activity.
Analytically, Ethyl-2-thiouracil is included in residue surveillance to detect non-compliant administration of antithyroid substances in food-producing animals.
Regulatory Status
Antithyroid agents of the thiouracil class are prohibited for use in food-producing animals in many jurisdictions (e.g., EU). Residues are subject to zero-tolerance policies or action-based decision criteria under veterinary drug control frameworks.
Competent authorities require validated methods (confirmatory LC–MS/MS) for enforcement. Laboratories should consult current national/EU/US regulations and guidance documents for decision limits and reporting protocols.
Monitoring and Surveillance
Targeted monitoring programs include Ethyl-2-thiouracil alongside other thiouracils (e.g., methyl- and propyl- derivatives) to distinguish exogenous administration from background thiouracil occurrence.
Results interpretation may consider species, diet (e.g., cruciferous feeds), matrix, and metabolite patterns. Confirmatory criteria typically include ion ratios, retention time matching, and, where applicable, metabolite profiling.
Target Matrices
- Animal urine (primary matrix for surveillance)
- Plasma/serum and tissues (liver, muscle)
- Feed and environmental samples when relevant to contamination studies
Analytical Methods
Instrumentation: LC–MS/MS with electrospray ionization is standard. Both reversed-phase and HILIC approaches are used depending on matrix and co-elution risks. Negative or positive ESI may be selected based on method optimization; negative mode is common for thiouracils due to acidic functionality.
Typical confirmation includes two MRM transitions with ion ratio tolerances and retention time matching to reference materials. Isotope-labelled internal standards are recommended for high-precision quantification and matrix effect compensation.
Sample Preparation
- Urine: dilution or SPE (e.g., mixed-mode or polymeric sorbents); optional enzymatic hydrolysis to address conjugates depending on the analytical scope.
- Tissues: homogenization, protein precipitation, liquid–liquid extraction or SPE cleanup; careful control of pH to preserve analyte stability.
- Method validation parameters: recovery, matrix effect, linearity, precision, trueness, carryover, stability (bench-top, freeze–thaw, autosampler), and measurement uncertainty.
Quality Control and Traceability
Use matrix-matched calibration and internal standards for quantification. Include system suitability, blanks, independent check standards, and spiked QC samples per batch. Employ traceable reference materials with certificates detailing purity, identity confirmation, and uncertainty budgets.
Health Impact — Human Toxicity
Mechanism: inhibition of thyroid hormone synthesis leading to decreased T3/T4. Potential effects include hypothyroidism-related symptoms upon sufficient exposure. Risk assessment in food safety is based on preventing consumer exposure through prohibition and residue control.
Laboratory handling risks are primarily chemical exposure via skin, ingestion, or inhalation of dust/aerosols; implement appropriate controls.
Environmental Impact — Effects on Wildlife
Thiouracils are polar and can be mobile in aqueous systems. While they may undergo degradation, inadvertent release could affect thyroid-mediated endocrine pathways in aquatic organisms. Good laboratory practices minimize environmental release.
Safety Measures
- Handle in a fume hood; avoid dust generation and inhalation.
- Wear lab coat, nitrile gloves, and eye protection.
- Store in tightly closed containers, dry, protected from light, typically at 2–8 °C unless otherwise specified in the certificate.
- Dispose of waste according to institutional and regulatory requirements.
Cross-Reactivity and Interferences
Structurally related thiouracils can co-elute or share MRM transitions if not chromatographically resolved. Matrix components (urea, salts) may suppress ionization in urine analyses. Use chromatographic separation, optimized MRM, and internal standards to mitigate interferences.
Reporting and Decision Rules
Apply validated identification points, ion ratio tolerances, and retention time windows. Establish laboratory-specific decision limits and cut-offs aligned with competent authority guidance. Document uncertainty and apply it to compliance assessment where required.
Storage and Stability
Neat materials: keep sealed, dry, and protected from light. Solutions: prepare with suitable solvents (e.g., methanol or water–organic mixtures), store refrigerated, and monitor for degradation per stability studies. Refer to the certificate for shelf life and requalification intervals.
Analytical Standards
HPC Standards supplies reference materials for Ethyl-2-thiouracil for calibration, system suitability, and quality control across a wide range of matrices. Options include neat materials and ready-to-use solutions at application-relevant concentrations.
On request, we provide stable isotope-labelled derivatives for internal standardization, customized concentrations, blends with related thiouracils, and matrix-matched materials to support method development and validation.
Related Compounds
Frequently co-monitored: 2-thiouracil, methylthiouracil, propylthiouracil, and other antithyroid agents. Including these in analytical panels improves discrimination between exogenous administration and background findings.
Documentation and Support
Each reference material is delivered with a comprehensive certificate detailing identity confirmation, purity, concentration (for solutions), uncertainty, and handling instructions. Our technical team supports method setup, validation planning, and selection of appropriate materials for your regulatory framework.