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Nifursol – Reference Materials for Residue Analysis
Nifursol High-Purity Reference Materials for Confident LC-MSMS Residue Control
Achieve traceable, regulation-ready results with HPC Standards certified reference materials for Nifursol and its marker metabolite DNSH. Our portfolioincl. isotopically labelled internal standardssupports method development, matrix-matched calibration, and confirmatory LCMSMS at sub-gkg levels across meat, dairy, eggs, honey, aquaculture, feed, and environmental samples. Produced under stringent QC to international requirements, each lot ships with comprehensive documentation for accreditation and audits. Rely on HPC Standards to streamline validation, ensure system suitability, and safeguard compliance in multi-residue nitrofuran monitoring.
Product | Catalog No./ CAS No. | Quantity | Price | |
|---|---|---|---|---|
 | 692996 | 1X5MG | Please log in. | |
13C6-Nifursol solution |  | 692997 | 1X1ML | |
 | 685429 | 1X100MG | Please log in. |
High-purity reference materials for Nifursol and its marker metabolite DNSH to support reliable LC-MS/MS residue monitoring in food and environmental matrices. Manufactured and quality-controlled to international requirements for regulatory compliance.
Overview
Nifursol is a nitrofuran-class veterinary medicinal compound that was historically used in food-producing animals. Due to concerns about genotoxic and carcinogenic properties of nitrofurans and their metabolites, Nifursol use is prohibited in many jurisdictions. Residue control focuses on the protein-bound marker metabolite DNSH in animal-derived foods and related matrices.
HPC Standards provides certified reference materials to support method development, system suitability, calibration, and quality control in confirmatory residue analysis.
Chemical Identity
Class: Nitrofurans (synthetic nitrofuran derivatives)
Primary residue marker: DNSH (3,5-dinitrosalicylic acid hydrazide), formed as a bound metabolite of Nifursol in animal tissues.
Typical analyte forms: Free and protein-bound residues; the latter require hydrolysis prior to analysis.
Uses (Historical/Veterinary)
Nifursol was used as an antimicrobial/coccidiostatic agent in certain food animals. Its use has been discontinued or banned in many regions because nitrofurans and their metabolites are considered genotoxic and potentially carcinogenic.
Regulatory Status
European Union and numerous other regions: Prohibited for use in food-producing animals. Control programs target marker metabolites of nitrofurans, including DNSH for Nifursol.
Action levels: Many authorities apply reference points for action (RPAs) or comparable decision limits for nitrofuran marker metabolites in the sub-µg/kg to low µg/kg range. Laboratories should verify the current national or regional threshold and validation requirements.
Method requirements often include LC-MS/MS confirmatory analysis with isotopically labelled internal standards and validated measurement uncertainty.
Monitoring and Compliance
Target matrices: Muscle, liver, kidney, eggs, milk, honey, aquaculture products, feed, and environmental samples potentially impacted by animal production.
Analyte target: DNSH as the marker metabolite for Nifursol. Surveillance programs typically monitor multiple nitrofuran markers in a single run (DNSH, AOZ, AMOZ, AHD, SEM).
Typical performance criteria: Low ng/g (µg/kg) limits of quantification, robust identification points, and retention time/ion ratio confirmation per regulatory guidelines.
Sample Preparation
Protein-bound residue determination generally requires acid hydrolysis to release the marker metabolite from tissue matrices.
Derivatization: Many validated methods for nitrofuran metabolites use on-line or off-line derivatization (e.g., with 2-nitrobenzaldehyde) to improve chromatographic behavior and MS response. Method specifics for DNSH may vary; follow the relevant official or validated procedure.
Clean-up: Solid-phase extraction or dispersive clean-up is commonly applied prior to LC-MS/MS to reduce matrix effects.
Analytical Methods
Instrumental: LC-MS/MS in MRM mode is the reference technique for specificity and sensitivity at regulatory levels.
Quantification: Matrix-matched calibration with isotopically labelled internal standards is recommended to correct for extraction and ionization effects.
Quality control: Include solvent and procedural blanks, fortified recovery samples, continuing calibration verification, and participation in proficiency testing where applicable.
Metabolites and Markers
Primary marker for Nifursol: DNSH (3,5-dinitrosalicylic acid hydrazide), typically measured after hydrolysis of protein-bound residues.
Multi-residue context: Routine monitoring often quantifies DNSH alongside AOZ, AMOZ, AHD, and SEM to screen for other nitrofuran exposures.
Health Impact
Nitrofurans and their metabolites have demonstrated genotoxic and carcinogenic potential in experimental studies. As a result, a no-tolerance approach is widely applied in food from treated animals, with action limits set at very low concentrations for the corresponding marker metabolites.
Environmental Impact
Historic veterinary use may result in environmental dissemination via manure and processing waste streams. Persistence of protein-bound residues and potential transformation products necessitates sensitive analytical monitoring in impacted matrices.
Safety Measures (Laboratory)
Handle Nifursol-related materials using appropriate PPE (lab coat, nitrile gloves, safety glasses) and work in a fume hood. Avoid skin contact and inhalation. Dispose of waste according to hazardous chemical and solvent regulations.
Prepare and store solutions with accurate weighing and volumetry to minimize uncertainty. Use amber glassware where photolability is a concern.
Storage and Stability
Store solid reference materials tightly closed, dry, protected from light, at recommended temperatures.
Prepare fresh solutions as needed and refrigerate or freeze aliquots per certificate instructions. Monitor solution stability and avoid repeated freeze–thaw cycles.
Analytical Reference Materials from HPC Standards
Available products include:
- Nifursol reference materials for qualitative/quantitative analysis
- DNSH (marker metabolite) reference materials
- Isotopically labelled internal standards (where available) for isotope-dilution LC-MS/MS
Each product is manufactured under stringent quality control and supplied with comprehensive documentation to support accreditation and regulatory audits.
Applications
- Confirmatory residue testing in official control and industry laboratories
- Method development and validation at sub-µg/kg levels
- Routine batch quality control, system suitability, and calibration verification
Why HPC Standards
HPC Standards GmbH specializes in reference materials for pesticides, veterinary drugs, their metabolites, and stable isotope-labelled derivatives. Our products are tested against international quality requirements and support compliant, traceable, and reproducible residue analysis in food and environmental laboratories.