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Spirotetramat-enol Reference Materials
Spirotetramat-enol Reference Materials Precision for Regulatory-Grade Residue Analysis
Ensure confident identification and quantification of spirotetramat-enol with high-purity reference materials from HPC Standards GmbH. Manufactured to international quality requirements, our standards deliver traceability, robust calibration, and reliable LCMSMS performance across challenging food and environmental matrices. Benefit from documented purity, matrix-matched calibration support, and optional stable isotopelabelled derivativestailored to help your laboratory meet SANTEISO criteria and ISOIEC 17025 accreditation needs. Choose HPC Standards for consistent results, secure compliance with global MRLs, and streamlined routine monitoring.
Product | Catalog No./ CAS No. | Quantity | Price | |
|---|---|---|---|---|
ISO 17034 Reference Material |  | 684780 | 1X10MG | Please log in. |
ISO 17034 Certified Reference Material |  | 693846 | 1X10MG | Please log in. |
High-quality reference materials for confident identification and quantification of spirotetramat-enol in food and environmental matrices. Manufactured by HPC Standards GmbH to international quality requirements for regulatory residue analysis.
Overview
Spirotetramat-enol (often referred to as S-enol) is the primary bioactive metabolite of the systemic insecticide spirotetramat (tetramic acid class). It forms in plants and is central to the overall insecticidal efficacy and residue profile.
Due to its polarity and mobility in both xylem and phloem, spirotetramat-enol is frequently included in residue definitions for monitoring and enforcement.
Chemical Identity
Class: Tetramic acid derivative; bioactive enol metabolite of spirotetramat.
Typical properties: more polar and more mobile than the parent compound; suitable for LC-based analysis.
Mode of Action
Spirotetramat and its enol metabolite inhibit lipid biosynthesis in target pests (Insecticide Resistance Action Committee Group 23). The enol metabolite is a key contributor to systemic efficacy.
Uses and Application Areas
Parent spirotetramat is used on fruit, vegetables, and ornamental crops against sucking pests (e.g., aphids, whiteflies, scale insects). Spirotetramat-enol is formed in planta post-application and is relevant for residue control in harvested commodities.
Residue Definitions and Metabolite Relevance
Regulatory residue definitions for spirotetramat commonly comprise the parent and specific metabolites, including spirotetramat-enol, expressed as spirotetramat. Laboratories often quantify spirotetramat-enol individually and/or as part of the sum for compliance assessment.
Regulatory Status and MRLs
Maximum residue limits (MRLs) for spirotetramat exist in many jurisdictions (e.g., EU, Codex, US). Enforcement typically targets the sum of spirotetramat and defined metabolites including spirotetramat-enol. Always consult the latest official databases for commodity-specific MRLs and residue definitions.
Monitoring and Surveillance
Food and environmental monitoring programs routinely screen for spirotetramat-enol alongside the parent and other metabolites. Multi-residue LC-MS/MS methods are commonly applied with LOQs at or below 0.01 mg/kg where feasible.
Sample Preparation
QuEChERS-based extractions (citrate/acetate buffering) and tailored clean-up (e.g., PSA/C18, SPE where needed) are widely used. Matrix effects can be significant; matrix-matched calibration and/or isotopically labelled internal standards are recommended.
Instrumental Analysis
Quantification is typically performed by LC-MS/MS in ESI mode using MRM transitions specific to spirotetramat-enol. Rugged chromatographic separation from co-extractives and related metabolites is critical to avoid interference and ensure accurate quantification.
Quality Assurance and Traceability
Accurate calibration requires traceable reference materials with well-characterized purity and uncertainty. Use independent calibration verification, system suitability checks, and participate in proficiency tests where available.
Health Impact – Human Toxicity
Available evaluations indicate low acute toxicity for spirotetramat and its key metabolites under normal handling and exposure conditions encountered in laboratories. Standard risk assessments consider dietary exposure relative to established MRLs and ADI/ARfD values for the parent compound. Follow institutional safety procedures to minimize exposure.
Occupational Safety Measures
Handle spirotetramat-enol reference materials in a fume hood. Wear appropriate PPE (lab coat, nitrile gloves, safety glasses). Avoid inhalation, ingestion, and skin/eye contact. Use calibrated pipettes, minimize aerosol formation, and dispose of waste according to local regulations.
Environmental Impact
As a metabolite arising from agricultural use of spirotetramat, spirotetramat-enol may be detected in plant tissues and environmental samples. Environmental risk assessments for the parent consider fate and effects of relevant metabolites, including potential impacts on aquatic organisms and non-target arthropods.
Effects on Pollinators and Wildlife
Risk characterizations for spirotetramat include evaluations for bees and beneficial insects; systemic movement may expose phloem-feeding arthropods. Adherence to label restrictions and good agricultural practice is essential to limit non-target exposure.
Storage and Stability
Store spirotetramat-enol reference materials tightly closed, protected from light, preferably at low temperature (e.g., 2–8 °C or -20 °C depending on the certificate). Prepare working solutions in suitable solvents (e.g., acetonitrile) and monitor stability per certificate of analysis.
Matrix Challenges and LOQs
Complex matrices (high water, sugar, acids, or lipids) may require tailored clean-up and matrix-matched calibration to achieve ≤0.01 mg/kg LOQs. Validate recovery, linearity, precision, and measurement uncertainty across target matrices.
Compliance and Accreditation Support
Method validation should align with SANTE/ISO requirements (e.g., trueness, precision, selectivity, LOQ). Use traceable reference materials and, where applicable, stable isotope-labelled internal standards to support ISO/IEC 17025 accreditation.
Related Metabolites
Other spirotetramat metabolites of regulatory relevance may include ketohydroxy, monohydroxy, ketoenol, and conjugates (e.g., enol-glucoside). Analytical methods should ensure specificity and appropriate summation consistent with the residue definition.
Analytical Standards and Reference Materials
HPC Standards GmbH manufactures and distributes spirotetramat-enol reference materials for calibration and quality control in residue analysis. Products are supplied with detailed documentation (purity, identity confirmation, traceability) to support reliable quantification.
Custom concentrations, solvent options, and stable isotope-labelled derivatives are available on request to optimize method performance and uncertainty budgets.
Why HPC Standards
Our reference materials are tested according to international quality requirements and meet the highest industrial standards. We specialize in pesticides, veterinary drugs, metabolites, and stable isotope-labelled derivatives, serving laboratories in food and environmental analysis to ensure compliance with regulatory limits.