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Fenbendazole-sulfone — Reference Materials for Veterinary Drug Residue Analysis
Fenbendazole-sulfone Precision Reference Materials for Confident Compliance
Ensure traceable, regulatory-grade quantification with high-purity Fenbendazole-sulfone from HPC Standards. Our certified reference materialsavailable as neat substances and ready-to-use solutions, including stable isotope-labelled optionsare engineered for LC-MSMS workflows across food and environmental matrices. Backed by comprehensive CoAs and validated to international quality requirements, we help laboratories achieve robust calibration, reliable recoveries, and defensible results for MRL compliance. Partner with HPC Standards for expert support in veterinary drug residue analysisfrom method development to accreditation.
Product | Catalog No./ CAS No. | Quantity | Price | |
|---|---|---|---|---|
 | 677092 | 1X10MG | Please log in. | |
Fenbendazole-sulfone solution |  | 682394 | 1X1ML | Please log in. |
High-purity reference materials for fenbendazole-sulfone to support reliable quantification in food and environmental monitoring, validated to international quality requirements.
Overview
Fenbendazole-sulfone is the oxidized metabolite of the benzimidazole anthelmintic fenbendazole. It forms via metabolic oxidation (following the sulfoxide, oxfendazole) in treated animals and can be present as a residue in edible tissues, milk, and eggs. It is a relevant target analyte in regulatory residue monitoring for veterinary medicinal products.
As a metabolite, fenbendazole-sulfone contributes to the total residue definition in many jurisdictions, necessitating robust, traceable quantification using validated analytical methods.
Chemical and Metabolic Profile
Class: Benzimidazole anthelmintic metabolite.
Biotransformation: Fenbendazole → sulfoxide (oxfendazole) → sulfone. The sulfone is generally more polar than the parent compound, impacting extraction and chromatographic behavior.
Physicochemical tendencies: Low aqueous solubility; good solubility in organic solvents (e.g., acetonitrile, methanol, DMSO). Compatible with reversed-phase LC separations.
Uses (Context in Veterinary Medicine)
Fenbendazole is used for the control of gastrointestinal parasites in food-producing animals (e.g., cattle, sheep, swine, poultry) and companion animals. Fenbendazole-sulfone is not administered directly but appears as a marker metabolite in residue depletion and monitoring studies.
Regulatory Landscape
Residues of fenbendazole and its metabolites, including fenbendazole-sulfone, are regulated under veterinary drug maximum residue limits (MRLs) or tolerances in many markets (e.g., EU, UK, US, Codex). The residue definition may include the parent compound and one or more metabolites in specified matrices.
Compliance requires validated analytical methods and traceable calibration using suitable reference materials. Always consult current regional regulations and compendia for matrix-specific MRLs and residue definitions.
Monitoring and Compliance
Typical monitored matrices: muscle, liver, kidney, fat, milk, eggs, and, where applicable, honey and aquaculture products.
Laboratory workflows often include screening (LC-MS/MS) followed by confirmatory quantification with internal standards. Stable isotope-labelled reference materials are recommended for correction of recovery and matrix effects.
Health Impact — Human Toxicity
At regulated levels, consumer exposure via food is controlled by MRLs, withdrawal periods, and surveillance. Benzimidazoles show low acute toxicity in humans but may exhibit developmental or hepatic effects in experimental systems at high exposures. Risk assessments underpin MRLs to ensure an adequate margin of safety.
Laboratory exposure should be minimized; avoid inhalation and dermal contact during handling of powders and solutions.
Environmental Impact
Excreted parent drug and metabolites can enter manure and the environment. Sorption to solids and low water solubility limit mobility, but localized exposure may occur. Potential effects on non-target organisms depend on concentration, matrix, and exposure duration.
Best practice includes proper waste management and avoidance of uncontrolled discharges from laboratories.
Safety Measures for Laboratory Handling
Handle in a chemical fume hood. Wear lab coat, nitrile gloves, and safety glasses. Avoid dust formation and aerosolization. Use calibrated pipettes for solutions. Dispose of waste according to institutional and local regulations.
First aid: Inhalation—fresh air; Skin—wash with soap and water; Eyes—rinse with water; Ingestion—rinse mouth, seek medical advice. Provide SDS to medical personnel.
Sample Preparation and Extraction
Common approaches: protein precipitation and liquid–liquid extraction or solid-phase extraction from animal tissues and milk. Use matrix-appropriate cleanup (e.g., SPE reversed phase or mixed-mode) to reduce ion suppression in LC-MS/MS.
For complex matrices (liver, egg), consider dispersive cleanup or orthogonal SPE steps. Incorporate isotope-labelled internal standards at the earliest step.
Analytical Methods
Preferred technique: UHPLC/LC-MS/MS with ESI+, MRM transitions for fenbendazole-sulfone and co-occurring residues (fenbendazole, oxfendazole). Verify retention time stability and ion ratio criteria per regulatory guidelines.
Calibration: multi-level matrix-matched curves with stable isotope-labelled internal standards. Validate selectivity, linearity, accuracy, precision, recovery, LOQ, carryover, and stability.
System suitability: assess peak symmetry, resolution from isobaric species, and absence of interferences in blank matrices.
Quality and Documentation
Use reference materials accompanied by a certificate of analysis (CoA) detailing purity, identification, concentration (for solutions), uncertainty, and traceability. Store and handle according to supplier specifications. Document lot numbers, preparation records, and calibration traceability in your quality system.
Storage and Stability
Recommended storage: cool, dry, protected from light; refrigerated or frozen for long-term stability of neat materials and solutions as specified on the CoA. Prepare working solutions in suitable solvents (e.g., acetonitrile or methanol) and store in amber vials. Assess short- and long-term stability and freeze–thaw robustness during method validation.
Matrices and Application Areas
Food: bovine, ovine, porcine, poultry, milk, eggs, fish where applicable.
Environmental: manure, slurry, agricultural soils; investigative studies on fate and occurrence.
Related Compounds and Metabolites
Parent and related residues: fenbendazole (parent), oxfendazole (sulfoxide), fenbendazole-sulfone. Other benzimidazoles may be included in multi-residue methods. Confirm chromatographic separation to avoid misidentification.
Reference Materials from HPC Standards
HPC Standards provides high-purity reference materials for fenbendazole-sulfone, including neat materials and ready-to-use solutions on request. Stable isotope-labelled derivatives are available to improve quantification accuracy and matrix compensation.
All products are tested according to international quality requirements and supplied with comprehensive documentation (CoA) to support accreditation and regulatory submissions.