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Semicarbazide hydrochloride — Reference Materials for Residue Analysis
Semicarbazide hydrochloride Precision Reference Materials for Confident Residue Compliance
Ensure traceable, defensible results in LCMSMS workflows with HPC Standards high-purity Semicarbazide hydrochloride SEMHCl reference materials. Designed for sub-gkg detection and robust method validation, our neat and solution standards support screening and confirmation of nitrofuran metabolites across challenging matricespoultry, aquaculture, eggs, honey, baby food, and packaging investigations. Each lot comes with full metrological documentation purityassay, uncertainty, traceability, storage, optional stable isotopelabelled internal standards, and proven stability for reliable calibration and QC. Partner with HPC Standards for compliant, reproducible data in ISOIEC 17025 laboratories.
Product | Catalog No./ CAS No. | Quantity | Price | |
|---|---|---|---|---|
 | 679150 | 1X10MG | Please log in. | |
13C, 15N2-Semicarbazide hydrochloride solution |  | 693341 | 1X1ML | Please log in. |
 | 676811 | 1X100MG | Please log in. |
Reliable reference materials for Semicarbazide hydrochloride to support precise residue monitoring, method validation, and regulatory compliance in food and environmental laboratories.
Overview
Semicarbazide hydrochloride (SEM·HCl) is the hydrochloride salt of semicarbazide, a small, highly polar hydrazide used as an analytical marker in residue analysis. In food control, semicarbazide is widely monitored as a marker metabolite of the prohibited veterinary drug nitrofurazone and as a potential contaminant originating from processing aids and certain food-contact materials.
Due to its reactivity and polarity, semicarbazide is commonly determined following derivatization and LC–MS/MS analysis at sub-µg/kg levels.
Chemical Identity
Chemical name: Semicarbazide hydrochloride
Synonyms: SEM·HCl; aminourea hydrochloride (salt)
CAS: 563-41-7 (commonly cited for the hydrochloride salt)
Molecular formula (salt): CH6N3O·HCl
Relative molecular mass (approx.): 127.5 g/mol
Properties: highly water-soluble; hygroscopic tendency; basic salt; light-sensitive degradation possible in solution.
Uses and Sources
- Analytical marker for nitrofurazone exposure in food-producing animals.
- Potential occurrence from azodicarbonamide-treated materials and other processing-related sources (e.g., gaskets/sealants), leading to food contamination concerns.
- Reagent and intermediate in research and analytical laboratories.
Occurrence in Food and Materials
- Animal-derived matrices (poultry, aquaculture, eggs, honey, gelatin/collagen) may be screened for semicarbazide as a nitrofuran metabolite marker.
- Baby foods, jarred products, and other sealed foods have been monitored historically for semicarbazide potentially migrating from certain food-contact materials.
- Levels of interest are typically in the low µg/kg (ppb) range and below.
Regulatory Landscape
- Nitrofurazone and other nitrofurans are prohibited for food-producing animals in the EU; enforcement relies on detection of characteristic metabolites such as semicarbazide.
- Many authorities apply performance requirements targeting decision limits around 1 µg/kg for nitrofuran metabolite methods, with confirmatory LC–MS/MS.
- Food-contact materials are regulated to control potential migration; semicarbazide may be included in specific monitoring programs under relevant national/EU frameworks.
Monitoring and Compliance
- Routine surveillance and confirmatory testing in accredited laboratories (ISO/IEC 17025) using validated LC–MS/MS methods.
- Method validation parameters typically include selectivity, linearity, recovery, precision, decision limit (CCα), detection capability (CCβ), measurement uncertainty, and matrix robustness.
- Participation in proficiency tests and use of fit-for-purpose reference materials are essential for defensible data.
Analytical Methods
- Preferred technique: LC–MS/MS following derivatization of semicarbazide (e.g., with 2-nitrobenzaldehyde to form a more MS-amenable derivative).
- Alternative approaches include direct analysis with hydrophilic interaction chromatography (HILIC) or ion-pair LC–MS/MS where applicable, though derivatization remains common for sensitivity and selectivity.
- Typical LOQs: ≤1 µg/kg depending on matrix and instrumentation; sub-µg/kg achievable in optimized workflows.
Sample Preparation and Matrices
- Common matrices: poultry tissue, fish/shrimp, eggs, honey, milk/gelatin, baby food, and other complex foods; occasional testing of food-contact materials and simulants.
- Workflows: protein-bound residue release (where applicable), derivatization, liquid–liquid extraction or SPE cleanup, LC–MS/MS quantification using stable isotope-labelled internal standards.
- Critical points: prevention of artifactual formation, control of reagent blanks, matrix-matched calibration, and recovery correction.
Reporting Units and Performance
- Units: µg/kg (ppb) for foods; µg/L for extracts/simulants.
- Quality criteria: calibration model with r² ≥0.99, recoveries typically 70–120% (matrix-dependent), precision (RSD) within method targets at LOQ, and defined measurement uncertainty on the certificate/report.
Health Impact — Human Toxicity
- Semicarbazide has shown genotoxic and developmental effects in certain experimental settings at high exposures; regulatory bodies apply precautionary control in foods.
- Occupational exposure may cause irritation; handle as harmful on contact/ingestion and avoid inhalation of dust or aerosols.
- Risk management in the food chain focuses on preventing sources of contamination and maintaining residues below enforcement limits.
Environmental Impact — Effects on Wildlife
- Targeted environmental monitoring is limited; primary concern is food-chain exposure. Good laboratory and waste management practices minimize environmental release.
- No specific ecotoxicity benchmarks are routinely applied; adherence to chemical handling and disposal regulations is recommended.
Safety Measures for Handling
- Use in a fume hood with appropriate PPE: lab coat, safety glasses, and chemical-resistant gloves.
- Avoid dust formation and moisture uptake; the hydrochloride salt is highly soluble and may be irritating.
- Dispose of waste according to local regulations for laboratory chemicals; prevent release to drains.
Storage and Stability
- Store sealed in a cool, dry, and dark place (typically 2–8 °C) to minimize degradation and moisture ingress.
- For solutions and derivatized forms, follow validated hold times and protect from light; verify stability with control charts.
- Use within the assigned shelf life; always consult the certificate for storage and stability guidance.
Packaging and Transport
- Supplied in inert, tightly sealed containers minimizing moisture and light exposure.
- Shipped under controlled conditions appropriate to the product’s stability profile; temperature control as specified on the documentation.
Reference Materials from HPC Standards
- Neat and solution reference materials for semicarbazide hydrochloride designed for calibration, system suitability, and quality control in LC–MS/MS methods.
- Options include matrix-free solutions and, where applicable, stable isotope-labelled internal standards to improve accuracy and correct for matrix effects.
- Each item is accompanied by a detailed certificate including purity/assay, uncertainty, traceability, and storage conditions.
Quality and Traceability
- Manufactured under stringent quality requirements with full documentation for metrological traceability.
- Assigned values with stated expanded uncertainties, homogeneity and stability considerations, and lot-specific data ensure reliable quantification.
- Suitable for method development, validation (linearity, recovery, precision), and routine QC.
Applications in Food and Environmental Labs
- Confirmation of compliance in control programs for nitrofuran residues.
- Screening and confirmatory analysis of baby food, animal products, honey, and packaging-related investigations.
- Support for root-cause analysis of unexpected findings through robust calibration and QC schemes.
Related Compounds and Metabolites
- Nitrofurazone metabolite (semicarbazide) monitored alongside other nitrofuran metabolites (e.g., AOZ, AMOZ, AHD) depending on scope.
- Derivatization reagents and isotope-labelled analogues available to support enhanced selectivity and quantification.
Why HPC Standards
- Expert partner for pesticides, veterinary drugs, metabolites, and stable isotope-labelled derivatives in food and environmental analysis.
- Comprehensive technical documentation, responsive support, and dependable supply for regulated testing workflows.
- Reference materials rigorously tested to meet high industrial and international quality requirements for defensible, compliant results.