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Indoleacetic Acid (Indole-3-acetic acid, IAA) – Reference Materials for Precise Quantification
Indoleacetic acid HighPurity Reference Materials for Confident Quantification
Achieve reproducible, regulator-ready results with HPC Standards Indoleacetic acid IAA reference materials and isotopelabelled derivatives. Our certified, highpurity IAA supports LCMSMS and HPLC workflows across plant physiology, food and feed testing, and environmental fate studies. Benefit from matrixmatched accuracy with IAA-d5 or 13C6IAA internal standards, comprehensive CoAs purity, identity, uncertainty, and formats from neat solids to readytouse solutions. Rely on proven quality, technical support, and custom concentrations to streamline method validation, routine QC, and compliant reporting.
Product | Catalog No./ CAS No. | Quantity | Price | |
|---|---|---|---|---|
ISO 17034 Reference Material |  | 679302 | 1X250MG | Please log in. |
3-Indoleacetic acid solution |  | 688460 | 1X5ML | Please log in. |
ISO 17034 Certified Reference Material |  | 692647 | 1X100MG | Please log in. |
 | 687729 | 1X10MG |
HPC Standards GmbH provides high-purity reference materials for Indoleacetic acid (IAA) and stable isotope‑labelled derivatives to support accurate quantification in food, environmental, and research laboratories.
Overview
Indoleacetic acid (IAA; Indole-3-acetic acid) is the principal auxin phytohormone that regulates cell elongation, apical dominance, root initiation, and tropic responses in plants. It occurs naturally in a wide range of plant tissues and can also be used as a plant growth regulator in research and certain agronomic applications.
CAS: 87-51-4 | Molecular formula: C10H9NO2 | Synonyms: Indole-3-acetic acid, 3-IAA.
Applications and Uses
- Quantification of endogenous IAA in plant tissues for plant physiology and agronomy studies.
- Quality control and formulation studies for plant growth regulator products.
- Monitoring of IAA levels in food/feed plant matrices to differentiate endogenous occurrence from exogenous application.
- Environmental fate and degradation studies in soil and water.
Regulatory Context
IAA is primarily recognized as a naturally occurring plant hormone. When used as a plant growth regulator, it may be subject to regional regulatory frameworks governing plant protection products or biostimulants. Laboratories should verify applicable requirements for registration, labeling, and residue assessment in their jurisdiction.
Because IAA is endogenous to plants, interpretation of residues in food matrices must account for natural background levels. Where applicable, follow regional guidance on differentiation of natural vs. applied sources and any tolerance or exemption provisions.
Monitoring and Target Matrices
- Plant matrices: leaves, stems, roots, seeds, fruits (fresh and processed).
- Environmental matrices: agricultural soils, irrigation/runoff water, sediments.
- Formulations and technical materials for product QC.
Analytical Methods
- LC-MS/MS is preferred for selectivity and sensitivity; typical ESI negative mode with MRM transitions for IAA and isotope-labelled internal standards.
- HPLC-UV/FLD can be used for higher concentration ranges or purified extracts.
- GC-MS may be applied following derivatization (e.g., methylation/silylation) to improve volatility and signal.
Sample Preparation
- Plant tissue extraction using acidified aqueous-organic solvents (e.g., methanol/water with formic or acetic acid) with cold conditions to minimize enzymatic degradation.
- Cleanup via SPE (e.g., reversed-phase, mixed-mode) to reduce matrix effects.
- Consider in-sample stabilization (acidification, antioxidants, low temperature) due to IAA lability and potential photodegradation.
Calibration and Quantification Strategy
- Use matrix-matched calibration and isotope-dilution for highest accuracy.
- Typical internal standards: indole-3-acetic acid-d5 or 13C6-indole-3-acetic acid.
- Evaluate recovery, linearity, LOQ, and matrix effects; include procedural blanks and fortified QC samples with each batch.
Quality Assurance
- Employ traceable reference materials with certified purity and identity.
- Review CoA parameters: purity (chromatographic), identity (NMR/HRMS), water content, residual solvents, and uncertainty.
- Implement ongoing calibration verification and participation in interlaboratory comparisons where available.
Health Impact – Human Toxicity
IAA exhibits low acute toxicity and is not known to be carcinogenic. Potential hazards include skin and eye irritation and respiratory irritation from dust. Data on chronic effects in humans are limited. Standard laboratory precautions are adequate for handling.
Environmental Impact
IAA is naturally occurring and is biodegraded by microorganisms in soil and water; it generally shows low persistence. Ecotoxicological risk is typically low at environmentally relevant concentrations, but local exposure from applications should still be evaluated within risk assessments.
Safety Measures and Handling
- Avoid exposure to light; use amber glassware and minimize air/oxidation.
- Handle under dry, cool conditions; use appropriate PPE (lab coat, gloves, eye protection).
- Prevent dust generation; use fume hood for weighing and solution preparation.
Storage and Stability
- Store solid IAA in tightly sealed, amber containers at 2–8 °C or frozen for extended stability.
- Prepare stock solutions in suitable solvents (e.g., methanol) under low light; store aliquots at ≤ -20 °C.
- Avoid repeated freeze–thaw cycles; monitor stability with system suitability checks.
Typical Specifications (Reference Materials)
- Purity: ≥98% (HPLC/GC).
- Identity confirmed by MS/NMR.
- Accompanied by Certificate of Analysis with batch-specific data and uncertainty.
- Available as neat material and as ready-to-use solutions on request.
Isotope-Labelled Derivatives
- Common internal standards: IAA-d5, IAA-13C6 for isotope-dilution LC-MS/MS.
- Optimized for co-elution and minimal isotopic interference; supplied with labeling pattern details and exact concentration for solutions.
Method Development Considerations
- Control for isobaric interferences from structurally related indole compounds.
- Optimize mobile phase pH and ion-pairing avoidance to maintain ionization efficiency.
- Validate extraction efficiency across plant tissues with different matrices (high sugar, pigment, lipid).
Compliance and Documentation
HPC Standards GmbH reference materials are produced and tested according to international quality requirements and meet high industrial standards. Products are delivered with batch documentation to support method validation, routine QC, and regulatory submissions.
Why HPC Standards GmbH
- Expertise in pesticides, veterinary compounds, metabolites, and stable isotope-labelled derivatives.
- Comprehensive portfolio for plant hormones and growth regulators, supporting food and environmental analysis.
- Reliable lead times, technical support for method setup, and custom solutions upon request.
Order Information
- Product forms: neat solid, ampouled solutions in acetonitrile or methanol, and custom concentrations.
- Packaging: amber glass vials/ampoules with tamper-evident seals; optional multipacks for high-throughput labs.
- Request quotes, bulk supply, and custom blends including isotope-labelled internal standards for multi-analyte methods.