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Fumonisin B1 (FB1) — Reference Materials for Food and Environmental Analysis
Fumonisin B1 High-Purity Reference Materials for Confident Compliance
Secure precise quantification and robust method validation with HPC Standards Fumonisin B1 reference materials. Trusted by accredited food, feed, and environmental laboratories, our FB1 neat solids, certified solutions, and isotope-labeled analogues deliver traceable accuracy for LCMSMS and HPLC workflows. Each batch is produced to international quality requirements and supplied with comprehensive certificates covering purity, uncertainty, stability, and storageso you can meet regulatory limits, streamline audits, and maintain consistent QC performance. Choose HPC Standards for reliable calibration, proficiency testing readiness, and reproducible results across challenging maize-based matrices and beyond.
Product | Catalog No./ CAS No. | Quantity | Price | |
|---|---|---|---|---|
ISO 17034 Certified Reference Material Fumonisin B1 solution |  | 682051 | 1X1ML | Please log in. |
ISO 17034 Certified Reference Material Fumonisin B1 solution |  | 682052 | 1X5ML | Please log in. |
HPC Standards GmbH provides high-purity reference materials for Fumonisin B1 to support accurate quantification, method validation, and routine quality control in food, feed, and environmental laboratories. All materials are produced and tested under international quality requirements and supplied with comprehensive documentation for regulatory compliance.
Overview
Fumonisin B1 (FB1) is a polar mycotoxin predominantly produced by Fusarium species (notably Fusarium verticillioides and Fusarium proliferatum). It frequently contaminates maize (corn) and maize-derived products, as well as sorghum and related commodities. FB1 disrupts sphingolipid metabolism by inhibiting ceramide synthase, leading to characteristic changes in sphingoid bases and their metabolites. It is the most abundant and toxic member of the fumonisin group (FB1–FB4).
Chemical Identity and Properties
Chemical name: (2S,2′S)-2,2′-[(1R,2S,3S,3′R,4R)-1,3,7,9,11,15-hexahydroxy-3-methyl-2-[(S)-1-methylbutyl]-4,14-dimethyl-2,3,4,5,6,10,11,12,13,14,15,16-dodecahydro-1H,7H,9H,11H,15H-2,5:9,12-diepoxy-1,7,9,11,15-pentaazacyclohexadeca-4,8,12-triene]-bis(butanedioic acid)
Common identifiers: FB1; CAS: 116355-83-0. Molecular formula: C34H59NO15; highly polar, water-soluble; non-volatile; thermally labile; sensitive to strong alkaline conditions. Often determined as the sum with FB2/FB3 in regulatory contexts.
Sources and Occurrence
Primary occurrence in maize kernels pre- and post-harvest under warm, humid conditions. Contamination is heterogeneous within lots. Additional occurrence in maize-based flours, grits, breakfast cereals, infant foods, beer (maize adjunct), and animal feed. Co-occurrence with other Fusarium toxins is common.
Uses
FB1 has no intentional industrial or agricultural use. Its relevance is exclusively as a contaminant (hazard) requiring monitoring and control in food and feed supply chains.
Regulatory Status
Jurisdictions (e.g., European Union, Codex Alimentarius, US FDA) set maximum levels or guidance values for fumonisins in food and feed, typically expressed as the sum of FB1 and FB2 (±FB3) and matrix-specific limits (e.g., unprocessed maize, maize intended for direct human consumption, processed maize-based foods, infant foods). The EU also prescribes sampling and performance criteria for mycotoxin analysis.
Laboratories should consult the latest texts (e.g., EU Regulation on maximum levels for contaminants in foodstuffs; sampling and analytical performance criteria; national feed guidance) to ensure current compliance.
Health Impact — Human Toxicity
Mode of action: Potent inhibitor of ceramide synthase (sphinganine/sphingosine N-acyltransferase), causing accumulation of free sphingoid bases and disruption of cell signaling and membrane integrity.
Adverse effects: Hepatotoxicity and nephrotoxicity in animal models; epidemiological associations with esophageal cancer in high-exposure populations. IARC classification: possibly carcinogenic to humans (Group 2B). No acceptable daily intake universally agreed; risk assessment based on benchmark dose modeling for liver and kidney endpoints and sphinganine-to-sphingosine (Sa/So) ratio as a biomarker.
Environmental and Animal Health Impact
FB1 affects animal health via contaminated feed: equine leukoencephalomalacia (horses), porcine pulmonary edema syndrome (pigs), and liver/kidney toxicity in several species. Environmental persistence is moderate; the compound is not a pesticide and is produced in planta or during storage by Fusarium spp. Good agricultural and storage practices reduce occurrence.
Exposure and Risk Management
Primary human exposure: consumption of contaminated maize-based foods. Risk mitigation includes resistant varieties, field management, timely harvest, proper drying, sorting, milling fractionation, and adherence to HACCP-based controls. Processing may reduce but does not reliably eliminate FB1.
Monitoring and Surveillance
Regulated commodities require structured sampling plans recognizing lot heterogeneity. Routine testing targets raw maize, intermediate fractions (bran, germ), finished foods, infant foods, and feed. Trend analysis includes seasonal and regional variability and co-occurrence with other mycotoxins.
Sampling and Sample Preparation
Representative incremental sampling and grinding to fine homogeneous particle size are critical. Extraction commonly uses mixtures such as acetonitrile–water or methanol–water. Cleanup may include solid-phase extraction (SPE), immunoaffinity columns, or dilute-and-shoot with matrix-matched calibration and isotopically labeled internal standards to control matrix effects.
Analytical Methods
- LC–MS/MS: Preferred quantification for sensitivity and selectivity; measures FB1 (± FB2/FB3) with isotopically labeled internal standards. Typical LOQs: low µg/kg range in foods.
- HPLC with fluorescence detection: After pre-column derivatization (e.g., OPA), suitable for many matrices with appropriate cleanup.
- Performance criteria: Validate for selectivity, linearity, trueness, precision, recovery, matrix effects, LOQ, and measurement uncertainty in line with applicable regulations and recognized guidelines (e.g., EU performance criteria and ISO/IEC 17025).
Reference Materials
HPC Standards GmbH supplies FB1 reference materials as neat solids and certified solutions, as well as mixtures (e.g., FB1/FB2/FB3) and stable isotope-labeled analogues for isotope dilution (e.g., 13C- or 15N-labeled FB1). Each lot is delivered with a certificate detailing purity/assay, solvent composition, expanded uncertainty, homogeneity, traceability, and storage instructions. Materials support calibration, system suitability, method validation, and ongoing quality control.
Quality Assurance and Compliance
Implement full metrological traceability using certified reference materials and isotopically labeled internal standards. Apply matrix-matched or standard addition calibration where needed. Participate in proficiency tests and use quality control charts (e.g., Shewhart, EWMA) for long-term performance. Document conformity assessment to current regulatory specifications for each batch analyzed.
Storage, Stability, and Handling
Store FB1 reference materials in tightly closed, inert, amber containers under recommended temperatures (typically 2–8 °C for solutions; frozen storage for long-term stability; protect from moisture and alkaline conditions). Allow to equilibrate to room temperature before opening to avoid condensation. Use calibrated volumetric equipment and record each opening. Observe expiry dates and requalification intervals.
Safety Measures
Handle FB1 as a toxic mycotoxin: avoid inhalation, ingestion, and skin contact. Work in a fume hood, wear appropriate PPE (lab coat, nitrile gloves, eye protection), and follow institutional SOPs. Dispose of waste according to local regulations. Consult the safety data sheet (SDS) for hazard and precautionary statements.
Applications in the Laboratory
- Calibration of LC–MS/MS and HPLC-FLD methods for FB1 (and sum of fumonisins).
- Method development and validation across diverse matrices (raw maize, cereal products, infant foods, feed).
- Recovery studies, spike-and-recovery experiments, and matrix effect assessments.
- Internal quality control (IQC), external proficiency testing, and confirmation analysis.
Regulatory Documentation Support
Certificates accompany each reference material with batch-specific data to facilitate method verification, uncertainty estimation, and audit readiness. HPC Standards GmbH supports technical inquiries related to selection, use, and documentation to meet regulatory audits and client specifications.
Why HPC Standards GmbH
We specialize in reference materials for contaminants including mycotoxins, pesticides, veterinary drugs, metabolites, and stable isotope-labeled derivatives. Our products meet stringent international quality requirements, enabling reliable, reproducible quantification and compliance in accredited laboratories worldwide.