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Hexadecanoic acid-methyl ester (Methyl palmitate) — Reference Materials
Hexadecanoic acid-methyl ester Precision Reference Materials for Confident FAME Analysis
Drive reliable quantification with high-purity Hexadecanoic acid-methyl ester methyl palmitate from HPC Standards. Our traceable reference materials and ready-to-use GC-FIDGC-MS solutions enable accurate calibration, system suitability, and method validation across food, feed, environmental, petrochemical, and biodiesel testing EN 1410314105. Each lot is identity-confirmed, purity-specified typically 98, and delivered with a CoA. Customize concentrations, solvent systems, or multi-component FAME mixes to fit your workflow. Depend on HPC Standards for compliant, reproducible results in regulated laboratories.
Product | Catalog No./ CAS No. | Quantity | Price | |
|---|---|---|---|---|
ISO 17034 Reference Material |  | 682032 | 1X250MG | Please log in. |
ISO 17034 Certified Reference Material |  | 693967 | 1X100MG | Please log in. |
High-purity reference materials for reliable quantification and method validation in food, feed, environmental, petrochemical, and biodiesel analysis.
Overview
Hexadecanoic acid-methyl ester, commonly known as methyl palmitate, is the methyl ester of palmitic acid (C17H34O2). It is a key component of fatty acid methyl ester (FAME) mixtures and is widely used as a marker and calibrant in chromatographic analysis.
HPC Standards GmbH supplies high-purity reference materials for methyl palmitate to support accurate identification, quantification, and quality control across regulated laboratories.
Synonyms and Identifiers
Synonyms: Methyl palmitate; Palmitic acid, methyl ester; Hexadecanoic acid, methyl ester
CAS: 112-39-0 | Molecular formula: C17H34O2 | Molecular weight: ~270.45 g/mol
Physicochemical Properties
Appearance: Colorless to pale-yellow waxy solid or liquid (temperature-dependent)
Melting point: ~30–32 °C | Boiling point: ~350 °C (1013 hPa)
Density: ~0.85 g/cm³ (20 °C) | Log Kow: high (strong hydrophobicity)
Water solubility: very low (<1 mg/L at 25 °C) | Vapor pressure: very low
Uses and Applications
- Calibration and system suitability in GC-FID/GC-MS FAME profiling of edible oils, fats, dairy, and biological matrices.
- Biodiesel quality assessment (FAME composition and ester content) in line with EN 14103/EN 14105 and related methods.
- Reference marker in petrochemical and environmental monitoring of lipid-derived compounds.
- Research in lipidomics, flavor/aroma chemistry, and materials/lubricants where saturated C16 FAME is required.
Regulatory Status
- Typically not classified as hazardous under GHS/CLP for pure substance; verify current SDS for product-specific classification.
- Not regulated as a dangerous good for transport (ADR/RID/IMDG/IATA), unless part of a formulated mixture with different classification.
- Commonly REACH-registered in the EU by manufacturers/importers; compliance requirements depend on tonnage band and use.
Monitoring and Analytical Methods
- Primary techniques: GC-FID and GC-MS; methyl palmitate serves both as an analyte and as a calibration/response factor standard in FAME analyses.
- Sample preparation: extraction of lipids (e.g., Soxhlet, LLE, SPE) followed by transesterification to FAMEs for total fatty acid profiling (food/feed). For biodiesel, direct dilution in suitable solvents per method recommendations.
- System suitability: retention time locking, resolution checks within C14–C18 FAME window; linear calibration across relevant concentration ranges with internal standards as required.
- Reference methodologies: EN 14103/EN 14105 (biodiesel), ISO/EN methods for FAME GC profiling of fats and oils.
Health Impact — Human Toxicity
- Low acute toxicity is generally reported for saturated long-chain FAMEs; limited irritation potential. Avoid inhalation of aerosols/mists and prolonged skin contact.
- Not known to be mutagenic or carcinogenic; handle according to good laboratory practice and consult the SDS for product-specific data.
Environmental Impact — Effects on Wildlife
- Readily biodegradable in aerobic conditions; low acute aquatic toxicity is typical for long-chain FAMEs.
- High hydrophobicity may lead to sorption to sediments and organic matter; bioaccumulation potential is mitigated by biodegradation and metabolic breakdown.
Safety Measures and Handling
- Use standard laboratory PPE (lab coat, safety glasses, suitable gloves). Work in a well-ventilated area or fume hood.
- Avoid release to the environment. Prevent contact with strong oxidizers. Manage spills with absorbent materials.
- Always refer to the SDS for detailed hazard information, first-aid measures, and exposure controls.
Storage and Stability
- Store tightly closed, protected from light and heat. Recommended storage: ambient to cool conditions; avoid repeated freeze–thaw around the melting point.
- Stable under recommended conditions. Opened containers should be handled to minimize contamination and oxidation.
Quality and Compliance
- HPC Standards reference materials are produced and tested according to international quality requirements and meet the highest industrial standards.
- Each lot is accompanied by a Certificate of Analysis (CoA) detailing identity confirmation (e.g., GC/MS), purity, uncertainty (where applicable), and storage guidance.
Analytical Reference Materials from HPC Standards
- Formats: neat material and ready-to-use solutions in high-purity solvents at defined concentrations for GC-FID/GC-MS.
- Purity: typically ≥98% (lot-specific); identity verified by chromatographic and spectrometric methods.
- Traceability: batch-specific documentation ensures reproducibility for calibration, system suitability, and method validation.
- Customization: bespoke concentrations, solvent systems, and multi-component FAME mixes available on request.
Applications in Residue and Quality Control Analysis
- Calibration of FAME profiles in foods (oils, fats, meat/dairy), feeds, and environmental/lipid extracts.
- Biodiesel QC: ester content, composition, and impurity profiling in line with industry standards (e.g., EN 14103/14105).
- Internal/external standard in method development, robustness testing, and inter-laboratory comparisons.
Ordering Information
- Packaging: ampoules or vials in multiple sizes to match method throughput.
- Documentation: CoA and safety data sheet supplied; technical support for method integration and stability inquiries.
- For quotations, bulk supply, or custom mixtures, contact HPC Standards technical sales.