New user? / Forgot your password?
0 Item | 0,00 €
Leucomalachite Green (LMG) – Reference Materials for Food and Environmental Residue Analysis
Leucomalachite green Precision Reference Materials for Confident Compliance
Secure ultra-trace accuracy in MGLMG monitoring with high-purity Leucomalachite green LMG reference materials from HPC Standards. Manufactured and tested to international quality requirements, our neat and certified solutions including stable isotopelabelled analogs deliver reproducible calibration, robust QC, and regulatory-ready results for food and environmental laboratories. Benefit from matrix-matched mixes, comprehensive CoAs, and expert technical support to streamline LC-MSMS workflows, control interconversion, and meet zero-tolerance decision limits with confidence.
Product | Catalog No./ CAS No. | Quantity | Price | |
|---|---|---|---|---|
ISO 17034 Reference Material |  | 676847 | 1X10MG | Please log in. |
 | 692727 | 1X10MG | Please log in. | |
ISO 17034 Certified Reference Material D6-Leucomalachite green solution |  | 692728 | 1X1ML | Please log in. |
 | 674880 | 1X100MG | Please log in. | |
Leucomalachite green solution |  | 693435 | 1X5ML | Please log in. |
HPC Standards GmbH supplies high-purity reference materials for Leucomalachite Green (LMG) and related compounds to support robust, compliant residue analysis in food and environmental laboratories. Our reference materials are produced and tested according to international quality requirements and meet the highest industrial standards.
Overview
Leucomalachite green (LMG) is the colorless, reduced metabolite of the triarylmethane dye malachite green (MG). In treated organisms, MG is readily reduced to LMG, which is more lipophilic and persistent. LMG is therefore the primary target analyte for monitoring illicit use of MG in aquaculture and for forensic applications.
Because only the cationic MG form is strongly colored, LMG remains largely colorless; however, both forms are analytically significant due to regulatory prohibitions in food-producing animals.
Chemical Identity
- Class: Triarylmethane compounds (reduced “leuco” form of malachite green)
- Key relationship: MG (parent, colored cation) ⇄ LMG (reduced, colorless metabolite)
- Properties of LMG: lipophilic (reported log P ~5.7 for leuco form), higher tissue retention than MG
Uses
- Historical/legacy use: MG as a dye for textiles and paper; as an antimicrobial and fungistat in aquaculture (e.g., against Saprolegnia) – now widely prohibited in food applications.
- Forensic application: LMG is used in colorimetric tests for latent blood detection (oxidation of LMG to colored MG in the presence of hemoglobin and hydrogen peroxide).
- Laboratory relevance: LMG is the predominant residue found in fish following MG exposure and is the critical target for regulatory control.
Regulatory Status
- United States: MG/LMG use in food-related applications has been banned since 1983; repeated import alerts have targeted seafood contaminated with MG/LMG residues.
- United Kingdom and other jurisdictions: Prohibited for use in food-producing animals; similar restrictions apply in multiple markets worldwide.
- Consequence: Zero-tolerance or effectively zero MRL policies are commonly applied; any confirmed detection in food products can trigger non-compliance actions, recalls, or import refusals.
Monitoring and Surveillance
- Target matrices: Fish and seafood (muscle/skin), aquaculture water, sediments, and processing waters; feed and equipment swabs where applicable.
- Analytes: Both MG and LMG should be monitored; LMG typically exhibits longer half-life in fish muscle and is the primary residue of concern.
- Action: Competent authorities and industry QA programs routinely apply LC-MS/MS screening and confirmatory methods with sub-µg/kg (ppb) detection capability.
Health Impact
- Human toxicity: MG is moderately toxic and a severe irritant; animal data indicate DNA adduct formation and increased lung adenomas with exposure to MG/LMG at high doses. LMG, as the persistent metabolite, is considered relevant for human dietary exposure when contaminated fish is consumed.
- Risk driver: Persistence and bioaccumulation potential of LMG in edible tissues increase the importance of strict control and sensitive analytical detection.
Environmental Impact
- Fate: MG can be transformed to LMG; both may adsorb to sediments. The leuco form’s lipophilicity supports persistence in biota relative to the parent dye.
- Concern: Unauthorized releases from aquaculture or processing can contaminate water bodies and sediments, necessitating environmental monitoring.
Effects on Wildlife
- Aquatic organisms: Historical antimicrobial use reflects biological activity; unintended exposure can affect non-target species and early life stages (e.g., fish eggs). Residues in the aquatic environment may pose chronic risks.
Safety Measures and Handling
- Laboratory handling: Use appropriate PPE (gloves, lab coat, eye protection) and handle standards in a fume hood. Avoid skin/eye contact and inhalation of powders/solvents.
- Storage: Store LMG reference materials as specified on the CoA/label (typically cool, dry, protected from light). Observe expiry and requalification intervals.
Analytical Methods
- Common techniques: LC-MS/MS is preferred for sensitivity and selectivity; HPLC-UV/Vis can be used with derivatization/oxidation steps but is less specific.
- Sample preparation: Solid–liquid extraction from fish muscle; cleanup via SPE (e.g., reversed phase, ion-pair) as per validated methods. Analyze both MG and LMG when required.
- Performance: Method LOQs often at sub-ppb to low-ppb levels. Include matrix-matched calibration, internal standards, and rigorous QC to control matrix effects.
Method Validation and Quality Control
- Validate per ISO/IEC 17025 and relevant EU/US guidance: specificity, linearity, recovery, precision, LOQ, and measurement uncertainty.
- QC: Use independent check standards, blanks, fortified samples, and proficiency testing where available. Document carryover control due to strong dye affinity.
Interferences and Cross-Contaminants
- Structural analogs and dye residues can co-elute or share fragments; optimize chromatographic separation and MRM transitions.
- Oxidation/conversion: MG/LMG interconversion can occur during sample prep; control pH, redox conditions, and light to preserve native analyte ratios.
Reporting and Compliance
- Report MG and LMG individually with confirmation criteria (ion ratios, retention time tolerances).
- Apply jurisdictional decision limits (e.g., screening target concentrations) and uncertainty budgets. Any confirmed detection in regulated products may indicate non-compliance.
Application Areas
- Food safety labs: Surveillance of aquaculture products (fish, eel, shrimp).
- Environmental labs: Monitoring of aquaculture sites, surface waters, sediments.
- Forensic labs: Blood detection assays using LMG oxidation to MG.
Reference Materials from HPC Standards
- Product scope: LMG neat materials and solution reference materials; MG and related metabolites on request; stable isotope-labelled derivatives to support isotope-dilution LC-MS/MS.
- Quality: Produced and tested according to international quality requirements; supplied with Certificate of Analysis (purity, identity, uncertainty, traceability, storage conditions).
- Customization: Matrix-matched spiking solutions and multi-analyte mixes for seafood and environmental applications available upon request.
Advantages of Using HPC Standards Reference Materials
- Enhanced method reliability: Accurate calibration and trueness verification for ultra-trace determination of LMG/MG.
- Regulatory alignment: Supports compliance with zero-tolerance policies and confirmatory method requirements.
- Operational efficiency: Ready-to-use concentrations minimize preparation errors and reduce turnaround time.
Storage and Stability
- Follow label instructions for temperature and light protection. Use amber vials for solutions. Minimize freeze–thaw cycles. Track opening dates and apply recommended shelf lives.
Documentation and Support
- Each reference material includes a detailed CoA. Technical support for method development, validation planning, and troubleshooting is available from our scientific team.
Synonyms and Identifiers
- Synonyms: Leuco-malachite green; reduced malachite green (LMG).
- Relationship: Primary metabolite of malachite green (MG), the cationic dye historically used in aquaculture (now widely prohibited in food uses).
Ordering Information
- Formats: Neat solid and certified solution reference materials at common calibration levels; isotope-labelled analogs available.
- Packaging: Sealed, low-adsorption containers with tamper-evident closure; documentation enclosed.
- Lead time: Many items available from stock; custom blends on request.