Hydroxy chlorpromazine Reference Materials
Hydroxy chlorpromazine — High-Purity Reference Materials for Confident Quantitation
Power your LC–MS/MS and HPLC workflows with traceable Hydroxy chlorpromazine reference materials from HPC Standards. Our high-purity neat and solution-grade standards—including specific positional isomers and stable isotope‑labelled options—enable selective method development, calibration, and QC across clinical/toxicology, pharmacokinetics, and environmental residue monitoring.
What you gain:
- Proven reliability: CoA with purity, identity (NMR/HRMS/LC), concentration, uncertainty, and SI traceability
- Method-ready performance: Isomer-resolved targets, matrix-matched flexibility, and custom concentrations on request
- Robust compliance: Produced under stringent international quality requirements (ISO-oriented practices)
- Practical durability: Guidance on light/oxidation sensitivity and storage for long-term stability
Partner with HPC Standards for precise, reproducible results—backed by expert technical support, flexible pack sizes, and secure global shipping.
Product | Catalog No./ CAS No. | Quantity | Price | |
|---|---|---|---|---|
ISO 17034 Reference Material |  | 692911 | 1X10MG | |
ISO 17034 Certified Reference Material 7-Hydroxy chlorpromazine solution |  | 692912 | 1X1ML | |
ISO 17034 Reference Material |  | 692915 | 1X10MG | |
ISO 17034 Certified Reference Material D6-7-Hydroxy chlorpromazine solution |  | 692916 | 1X1ML |
High-purity reference materials for Hydroxy chlorpromazine and related metabolites to support accurate quantification in pharmaceutical, clinical, and environmental residue analysis.
Overview
Hydroxy chlorpromazine refers to hydroxylated metabolites of the antipsychotic drug chlorpromazine. These phase I metabolites (e.g., 7-hydroxychlorpromazine among other positional isomers) are relevant targets in pharmacokinetic studies, therapeutic drug monitoring (TDM), forensic toxicology, and environmental monitoring of pharmaceuticals in water cycles.
HPC Standards GmbH provides traceable reference materials for Hydroxy chlorpromazine to ensure reliable method development, calibration, and quality control in LC–MS/MS and HPLC assays.
Chemical Identity and Synonyms
- Typical class: Hydroxylated phenothiazine derivative (metabolite of chlorpromazine)
- General formula (example isomers): C17H19ClN2OS
- Synonyms: Hydroxychlorpromazine; Hydroxylated chlorpromazine; CPZ hydroxy metabolite (positional isomers such as 7-hydroxychlorpromazine)
- Note: Multiple positional isomers may occur; specify the exact isomer for method selectivity.
Uses and Origin
Chlorpromazine is a phenothiazine antipsychotic used in human medicine; hydroxylated metabolites form via hepatic CYP-mediated oxidation. Hydroxy chlorpromazine is measured to evaluate drug metabolism, patient compliance, exposure, and environmental release via wastewater effluents.
Formation and Metabolic Pathways
- Primary route: Cytochrome P450 hydroxylation of the chlorpromazine tricyclic system and/or side chain.
- Conjugation: Subsequent phase II reactions (e.g., glucuronidation or sulfation) can occur; however, free (unconjugated) hydroxy metabolites are typical analytical targets after deconjugation or direct analysis.
Regulatory and Compliance Context
- Pharmaceutical residue monitoring: Hydroxy chlorpromazine and parent drug may be included in national or regional watch lists for pharmaceuticals in surface water and wastewater.
- Food matrices: No established MRLs for Hydroxy chlorpromazine (not a veterinary-approved residue marker). Laboratories may screen in food only within research or non-target assessments.
- Clinical/toxicology: Applied in TDM/forensic workflows per laboratory-developed test procedures aligned with GLP/GCP/ISO 17025/ISO 15189 as applicable.
Monitoring and Target Matrices
- Environmental: Influent/effluent wastewater, surface water, groundwater, sludge, sediments.
- Biological/clinical: Plasma, serum, whole blood, urine, hair.
- Forensic/toxicology: Postmortem matrices, DBS/volumetric microsampling where validated.
Analytical Methods
- Preferred: LC–MS/MS or LC–HRMS for isomer resolution and sensitivity (ng/L–ng/mL range).
- Sample prep: SPE (mixed-mode, reversed-phase), protein precipitation for clinical matrices, dilute-and-shoot for wastewater with matrix-matched calibration.
- Chromatography: C18 or polar-embedded phases; consider isomer separation; mobile phases with volatile buffers (e.g., ammonium formate) and formic acid.
- Detection: Positive ESI; monitor characteristic product ions for each isomer; confirm with qualifier transitions or exact mass/fragment ratios.
Method Validation Considerations
- Selectivity for positional isomers and possible interferences from parent chlorpromazine and other phenothiazines.
- Linearity across relevant ranges (environmental: low ng/L; clinical: low–mid ng/mL).
- Accuracy/precision per ISO/ICH/validation guidelines; matrix effects assessed via post-extraction addition.
- Recovery and process efficiency with internal standards; stability under light and during autosampler residence.
Stability and Handling
- Light sensitivity: Phenothiazines and their hydroxy metabolites can undergo photodegradation; protect from light (amber vials).
- Chemical stability: Susceptible to oxidation; minimize air exposure, use inert caps, store at low temperature.
- Recommended storage: ≤ -20 °C for stock solutions/solids; short-term at 2–8 °C; follow CoA instructions.
Health Impact
- Human toxicity: Hydroxy chlorpromazine exhibits pharmacological activity related to the parent drug (dopaminergic antagonism; potential anticholinergic and antihistaminic effects). Overexposure may cause CNS depression, hypotension, anticholinergic symptoms, and photosensitivity.
- Laboratory exposure: Risk via dermal/ocular contact or inhalation of aerosols/solvents; handle using appropriate engineering controls and PPE.
Environmental Impact
- Occurrence: Detected as a transformation product of chlorpromazine in wastewater and surface waters.
- Ecotoxicology: Potential effects on aquatic organisms; photoreactivity can generate additional transformation products; biodegradation may be limited depending on conditions.
- Risk assessment: Part of broader pharmaceutical micropollutant surveillance; apply effect-directed analysis where relevant.
Safety Measures
- PPE: Laboratory coat, nitrile gloves, safety glasses; avoid skin contact and inhalation.
- Work under subdued light or amber shielding; use ventilated enclosures for solution prep.
- Waste: Dispose as hazardous pharmaceutical/organic waste according to local regulations.
Analytical Reference Materials from HPC Standards
- Product types: Neat materials and ready-to-use solutions for specific Hydroxy chlorpromazine isomers (where available), plus parent chlorpromazine and related metabolites for method suites.
- Isotopically labelled options: Stable isotope-labelled internal standards available for selected targets to improve quantitation and matrix effect correction.
- Concentration ranges: Custom concentrations and solvent systems on request to fit your calibration strategy.
Quality and Documentation
- Full Certificate of Analysis (CoA) with purity, identity (NMR/HRMS/LC), concentration (for solutions), uncertainty, and traceability to SI where applicable.
- Manufactured under stringent quality controls compliant with international requirements; batch-specific stability information provided.
Applications
- Clinical/toxicology: TDM, adherence studies, forensic casework.
- Environmental: Wastewater-based epidemiology, WWTP performance studies, surface water monitoring.
- Method development: Isomer-resolved quantitation, confirmation of transformation pathways, research on photolytic/biotic degradation.
Related Compounds
- Parent: Chlorpromazine.
- Other metabolites: Desmethyl chlorpromazine, didesmethyl chlorpromazine, sulfoxide derivatives, conjugated forms (for deconjugation studies).
Ordering and Support
- Flexible pack sizes for screening to routine QC.
- Technical support for method setup, isomer selection, and matrix challenges.
- Global shipping with temperature and light protection as required.