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Leucobrilliant Green (LBG) Reference Materials
Leucobrilliant green Precision Reference Materials for Confident Residue Control
Ensure regulatory-ready results with validated Leucobrilliant Green LBG reference materials from HPC Standards. Our high-purity neat and solution standards, including optional isotope-labelled derivatives, enable sensitive LC-MSMS and LC-FLDUV workflows for LBGBG in seafood, aquaculture water, and environmental matrices. Delivered with a comprehensive CoA purity, concentration, uncertainty, traceability, our materials support method development, routine monitoring, and proficiency testinghelping accredited labs achieve low LOQs, robust QAQC, and defensible decisions. Custom concentrations, mixtures, and matrix-matched options available on request.
Product | Catalog No./ CAS No. | Quantity | Price | |
|---|---|---|---|---|
 | 689456 | 1X10MG | Please log in. | |
 | 689223 | 1X10MG | Please log in. | |
Leucobrilliant green solution |  | 692348 | 1X1ML | Please log in. |
High-quality reference materials for precise identification and quantification of Leucobrilliant Green and related analytes in food and environmental matrices.
Overview
Leucobrilliant Green (LBG) is the reduced, colorless leuco-form of the triphenylmethane dye Brilliant Green (BG). In residues analysis, LBG is a key target as a metabolite and marker of illegal or unintended use of Brilliant Green in aquaculture and related applications. Due to its higher lipophilicity and persistence compared to the parent dye, LBG can accumulate in fish and other biota, necessitating sensitive and selective monitoring.
HPC Standards GmbH supplies validated reference materials for LBG to support regulatory compliance, method development, routine monitoring, and proficiency testing in accredited laboratories.
Chemical Identity and Structure
- Synonyms: Leuco Brilliant Green, LBG; reduced Brilliant Green
- Chemical class: Triphenylmethane dye (leuco form)
- Relationship: Reduced metabolite/transform product of Brilliant Green (BG); can be oxidized back to BG during analysis.
Uses and Sources
- Historical: Cationic dye used in textiles, microbiology (selective media), and antiseptic applications.
- Illicit/legacy use: Formerly used in aquaculture as an antifungal/antiparasitic agent; current food-production use is widely prohibited.
- Residue relevance: LBG forms in vivo and in the environment from BG and is often the predominant residue in fish tissues.
Regulatory Status
- EU: Brilliant Green and its leuco-form are not permitted for use in food-producing animals; monitoring follows reference points for action (e.g., historically 2 µg/kg for analogous triphenylmethane dyes in aquaculture products). Laboratories should verify current requirements under Regulation (EU) 2017/625 and (EU) 2021/808 and national implementing acts.
- USA: No approved use in food fish; agencies apply zero tolerance with enforcement action levels set by method capability.
- International: Many jurisdictions treat BG/LBG similarly to malachite green/leucomalachite green with stringent residue controls in seafood and aquaculture products.
Monitoring Priorities and Matrices
- Primary matrices: Fish and seafood (muscle, skin, liver), aquaculture water, sediments, feeds.
- Complementary matrices: Processing water, equipment swabs, by-products, and environmental samples near aquaculture sites.
- Analyte scope: LBG, BG, and related triphenylmethane dyes; sum of parent plus leuco-form is commonly reported.
Health Impact
- Risk context: Triphenylmethane dyes and their leuco-forms have been associated with mutagenic and potential carcinogenic effects in experimental systems; precautionary regulatory controls apply.
- Human exposure: Primarily via consumption of contaminated seafood; stringent monitoring aims at non-detectable levels in food.
Environmental Impact
- Persistence: Leuco forms are more lipophilic and can persist in biota and sediments.
- Fate: LBG interconverts with BG under oxidative conditions; photolysis and microbial processes influence distribution.
Effects on Wildlife
- Aquatic toxicity: Triphenylmethane dyes can be toxic to fish and invertebrates; sublethal effects include enzyme inhibition and oxidative stress.
- Bioaccumulation: LBG may bioaccumulate due to higher affinity for lipids relative to the parent dye.
Safety Measures and Handling
- Laboratory PPE: Lab coat, nitrile gloves, safety glasses; handle solutions in a fume hood.
- Light/oxidation sensitivity: Protect from light and air; minimize exposure to oxidants to prevent conversion to BG.
- Waste: Collect dye-containing waste as hazardous according to local regulations.
Physicochemical Considerations
- Form: Reduced, colorless species; more nonpolar than BG.
- Stability: Prone to oxidation to BG; stability improved under inert atmosphere, low temperature, and subdued light.
- Solubility: Good solubility in organic solvents (e.g., acetonitrile, methanol); limited aqueous stability under oxidative conditions.
Analytical Methods
- LC-MS/MS: Gold-standard for selective quantification of LBG and BG (multiple reaction monitoring; ESI+ for BG, ESI± optimization for LBG depending on derivatization/ionization strategy).
- LC-FLD/UV: Employed after on-line or off-line oxidation of LBG to BG to exploit the strong chromophore/fluorophore of the parent dye.
- Confirmatory approach: Dual-analyte confirmation (LBG and BG) and ion ratio/retention time criteria per ISO/IEC 17025 and EU decision rules.
Sample Preparation and Cleanup
- Extraction: Solvent extraction from fish tissue (e.g., acetonitrile or acetonitrile–buffer systems), often with acid modifiers.
- Cleanup: Solid-phase extraction (SPE; strong cation exchange or reversed-phase) to reduce matrix effects.
- Oxidation step (optional): Controlled oxidation of LBG to BG for LC-FLD/UV workflows; maintain validated conversion efficiency and correction factors.
Calibration, QA/QC, and Traceability
- Calibration: Matrix-matched or standard addition calibration for LC-MS/MS; bracketing with multi-level calibrators.
- Internal standards: Prefer stable isotope-labelled derivatives of LBG/BG for recovery correction and matrix-effect compensation.
- QC: Procedural blanks, fortified matrix controls at decision limits, and ongoing precision and recovery checks per SANTE/ISO guidelines.
Reporting and Decision Limits
- Results: Report individual concentrations of LBG and BG and, where required, the sum (as BG equivalents if oxidation is applied).
- Limits: Align method LOQ with applicable regulatory reference points/action limits; maintain measurement uncertainty budgets to support compliance decisions.
Storage and Stability
- Reference material storage: Keep sealed in amber containers at 2–8 °C or per CoA; for long-term, -20 °C recommended.
- Solution stability: Prepare working solutions in oxygen-poor, amber vials; minimize headspace; verify stability with control charts.
- Light/air: Protect from light and air to prevent oxidation to BG.
Interferences and Cross-Reactivity
- Structural analogs: Malachite green/leucomalachite green and other triphenylmethane dyes can co-elute; ensure chromatographic resolution and unique MS/MS transitions.
- Matrix effects: High-lipid tissues may cause ion suppression; apply cleanup and isotope dilution.
Related Analytes and Metabolites
- Parent dye: Brilliant Green (BG)
- Analogous systems: Malachite Green (MG) and Leucomalachite Green (LMG)
- Oxidation products: Carbinol bases and demethylated species may appear; assess during method validation.
HPC Standards Reference Materials
- Portfolio: Neat and solution reference materials for Leucobrilliant Green and Brilliant Green; optional stable isotope-labelled derivatives for internal standardization.
- Quality: Manufactured under stringent quality requirements; supplied with Certificate of Analysis (CoA) detailing purity, concentration, uncertainty, and traceability.
- Formats: Convenient concentrations in acetonitrile or methanol; custom concentrations, mixtures (LBG/BG), and matrix-matched materials available on request.
Applications in Laboratories
- Routine surveillance of aquaculture products for banned dyes.
- Confirmation testing and enforcement support in official control laboratories.
- Method development/validation, proficiency testing, and instrument performance verification.