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Nandrolone – Reference Materials for Residue and Doping Analysis
Nandrolone Precision Reference Materials for Confident Residue and Doping Analysis
Strengthen your LCMSMS and GCMSMS workflows with high-purity nandrolone reference materials from HPC Standards. Our portfolio covers parent nandrolone and key metabolites 19-norandrosterone, 19-noretiocholanolone, 5-dihydronandrolone, including stable isotopelabelled analogues for robust isotope dilution. Achieve trace-level calibration, uncompromising selectivity, and ISOIEC 17025-aligned confidence across urine, serum, food matrices, and environmental waters. Available as neat materials or ready-to-use solutions, with custom blends and full documentation to meet international quality requirements. Rely on HPC Standardsyour expert partner for compliant, reproducible results in anti-doping, veterinary residue control, clinical, and environmental analysis.
Product | Catalog No./ CAS No. | Quantity | Price | |
|---|---|---|---|---|
 | 687333 | 1X5MG | Please log in. | |
D3-Nandrolone solution |  | 690077 | 1X1ML | Please log in. |
 | 676912 | 1X250MG | Please log in. | |
Nandrolone solution |  | 693479 | 1X5ML |
High-quality reference materials for nandrolone and key metabolites to support robust calibration, method validation, and regulatory compliance in food, environmental, clinical, and anti-doping laboratories.
Overview
Nandrolone (19-nortestosterone) is an endogenous androgen and an anabolic steroid used medically as long-acting esters (e.g., nandrolone decanoate, nandrolone phenylpropionate). It is also a controlled substance due to performance-enhancing misuse. Trace endogenous occurrence and extensive metabolism make accurate analytical differentiation between exogenous administration and background levels essential.
Key identifiers: CAS 434-22-0; Molecular formula C18H26O2; Molar mass 274.40 g/mol. Common analytical targets include nandrolone and its metabolites 19-norandrosterone and 19-noretiocholanolone, as well as 5α-dihydronandrolone (DHN).
Uses
Medical: Historically indicated for anemia, cachexia, osteoporosis, and certain oncology settings via intramuscular or subcutaneous administration of nandrolone esters.
Non-medical: Misuse for physique/performance enhancement by athletes and bodybuilders. This misuse drives stringent monitoring programs in sports anti-doping and workplace testing.
Regulatory Status
Controlled substance: Classified as Schedule III (US), Schedule IV (CA), Class C (UK), and controlled in many other jurisdictions. Non-medical possession and distribution are generally illegal.
Food-producing animals: Use as a growth promoter is prohibited in the EU and many regions; residues are subject to strict regulatory oversight.
Monitoring and Surveillance
Matrices: Urine and serum/plasma (anti-doping and clinical); hair (retrospective exposure); food matrices (meat, liver, kidney) for veterinary drug residue control; environmental waters/wastewater for steroid contamination studies.
Analytes: Parent nandrolone; metabolites 19-norandrosterone and 19-noretiocholanolone; DHN. Isotope-ratio insights and steroid profiles are used to infer exogenous administration.
Health Impact
Human toxicity: Dose-dependent androgenic/progestogenic effects. Adverse effects include virilization (e.g., acne, hirsutism, voice deepening), menstrual disturbances, and potential hypogonadism/infertility with misuse. Compared to testosterone, nandrolone shows a higher anabolic:androgenic activity ratio but still presents significant androgenic risk on prolonged or high-dose exposure.
Environmental Impact
Steroid androgens can occur as micropollutants in aquatic systems. Nandrolone and metabolites may exert endocrine activity in non-target organisms, warranting monitoring in influent/effluent and receiving waters.
Safety Measures
Laboratory handling: Use standard precautions for controlled substances and hormones (PPE, containment, documented chain of custody). Dispose of solutions/solid waste per local regulations for pharmaceutical/steroid waste.
Reference material use: Avoid contamination when preparing low-level calibrators; dedicate glassware and use solvent blanks and matrix blanks to control carryover.
Analytical Challenges
Endogenous background and interindividual variability require high selectivity and stable isotope dilution for accurate quantitation. Isomeric metabolites and conjugates necessitate optimized hydrolysis and chromatographic separation.
Decision limits in anti-doping rely on precise identification (MRM transitions, ion ratios, retention time matching) and confirmation criteria per accreditation standards.
Analytical Methods
Typical workflows: Enzymatic hydrolysis of conjugates (urine), liquid–liquid or solid-phase extraction, and LC–MS/MS or GC–MS/MS with derivatization for steroid panels. High-resolution MS can enhance selectivity and confirmatory analysis.
Performance goals: Sub-ng/mL LOQs in urine/serum; low µg/kg in food matrices; ng/L in environmental waters. Include matrix-matched calibration and recovery-corrected quantitation.
Target Metabolites and Markers
Primary urinary markers: 19-norandrosterone and 19-noretiocholanolone (free and conjugated). DHN may be relevant for mechanistic interpretation. Monitoring metabolite ratios supports source attribution and result interpretation.
Quality Assurance and Accreditation
Method validation should cover selectivity, linearity, accuracy, precision, matrix effects, recovery, carryover, stability, and measurement uncertainty. Participation in proficiency testing and use of traceable reference materials support ISO/IEC 17025 compliance.
Regulation and Compliance
Anti-doping: Laboratories follow stringent identification/confirmation criteria and decision rules defined by relevant authorities. Accurate quantification of nandrolone metabolites and adherence to confirmatory protocols are mandatory.
Food control: Compliance with regional prohibitions and residue monitoring plans requires validated methods, confirmatory MS, and reliable calibration with appropriate reference materials.
Reference Materials
HPC Standards provides high-purity reference materials for nandrolone and its key metabolites (e.g., 19-norandrosterone, 19-noretiocholanolone, 5α-dihydronandrolone), including stable isotope-labelled analogues for isotope dilution mass spectrometry.
Product formats: Neat materials and ready-to-use solutions at defined concentrations; custom blends for steroid panels; matrix-appropriate solvents; documentation compliant with international quality requirements.
Product Benefits for Laboratories
Accurate calibration at trace levels; improved selectivity and quantitation via isotope-labelled internal standards; reduced uncertainty for confirmatory analysis; compatibility with LC–MS/MS and GC–MS/MS workflows.
Stability, Storage, and Handling
Store according to the certificate (typically refrigerated or frozen, protected from light). Verify stability and re-test intervals. Use amber vials and minimize evaporation and adsorption by using low-adsorption consumables.
Method Development Support
Recommended to establish matrix-matched calibration, evaluate enzymatic hydrolysis efficiency (urine), and optimize chromatographic separation of epimers/isobars. Include isotopically labelled internal standards for each target where possible.
Applications
Anti-doping testing, clinical/toxicological assessment, veterinary drug residue surveillance in food, and environmental monitoring of endocrine-active steroids.
Related Products
Reference materials for additional anabolic steroids and metabolites, steroid hormone panels, hydrolysis enzymes, and quality control samples to support full method validation and routine QA/QC.
Ordering and Support
Contact HPC Standards for technical data sheets, certificates, custom concentrations, bulk supply, and expert support for method development in food and environmental analysis, clinical toxicology, and anti-doping laboratories.