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Dicamba-5-hydroxy
Dicamba-5-hydroxy Precision Reference Standards for Confident LC-MSMS Quantification
Ensure defensible results across food, feed, water, and environmental matrices with HPC Standards high-purity Dicamba-5-hydroxy reference materials. Manufactured to international quality requirements, our traceable neat materials and ready-to-use solutions support ISOIEC 17025 and 17034 compliant workflowsideal for calibration, method validation, and routine QAQC.
What you gain
- Reliable quantification of a key dicamba metabolite in LC-MSMS negative ESI
- Lot-specific certificates with purity, identity, and uncertainty data
- Optional isotope-labelled derivatives for isotope dilution accuracy
- Custom mixes, concentrations, and solvents for your exact scope
Partner with HPC Standards GmbHyour expert in pesticide metabolites and isotope-labelled derivatives for food and environmental analysis.
Product | Catalog No./ CAS No. | Quantity | Price | |
|---|---|---|---|---|
 | 678117 | 1X25MG | Please log in. |
High-quality reference materials for Dicamba-5-hydroxy to support precise quantification in food, feed, water, and environmental residue analysis.
Overview
Dicamba-5-hydroxy is a hydroxylated metabolite of the benzoic acid herbicide dicamba. It forms through oxidative transformation (hydroxylation) in plants and environmental compartments and is relevant as a marker of dicamba exposure and degradation. Due to its acidic functionality, Dicamba-5-hydroxy exhibits high polarity and is typically analyzed using LC-MS/MS under negative electrospray conditions.
In residue programs, Dicamba-5-hydroxy may be included in monitoring scopes to assess dicamba use, off-target movement, and environmental fate. Robust reference materials are essential for accurate calibration, method validation, and quality control.
Uses
- Analytical target for residue analysis supporting herbicide use assessments.
- Metabolite marker in plant metabolism, soil dissipation, and water monitoring studies.
- Analyte for drift investigations and buffer-zone compliance evaluations.
Regulatory Context
Regulatory frameworks (e.g., EU, US, and other jurisdictions) set maximum residue limits (MRLs) for dicamba in food and feed commodities. Depending on jurisdiction and commodity, residue definitions for enforcement or risk assessment may include dicamba and one or more metabolites. Dicamba-5-hydroxy can be relevant in plant metabolism and environmental fate dossiers and may be included in surveillance programs to characterize residue profiles.
Laboratories should consult current national and international regulations to confirm whether Dicamba-5-hydroxy is included in residue definitions or monitoring lists and to align reporting with the applicable MRL, LOQ, and identification criteria.
Monitoring
- Matrices: high-water content crops, cereals, oilseeds, leafy vegetables, soil, groundwater/surface water, and air-deposition samples (dust/wipe extracts).
- Sample preparation: QuEChERS (acidified), solid-phase extraction (weak anion exchange or mixed-mode), or direct aqueous injection for water after filtration and pH control.
- Detection: LC-MS/MS (negative ESI), with isotope dilution where available to correct for matrix effects and recovery variability.
Health Impact
Available toxicological evaluations for dicamba indicate low to moderate acute toxicity and a safety profile consistent with approved agricultural uses when applied according to label directions. Metabolites such as Dicamba-5-hydroxy are generally considered to have equal or lower toxicity than the parent, but risk assessments remain jurisdiction-specific. Exposure in the analytical laboratory should be minimized by established chemical hygiene practices.
Human Toxicity
- Expected low acute toxicity; avoid ingestion, inhalation of aerosols, and skin/eye contact.
- Use appropriate personal protective equipment (PPE) and handle solutions in a fume hood.
- Follow institutional exposure control limits and safety data guidance for acidic herbicide metabolites.
Environmental Impact
Dicamba is mobile in soils and may reach surface and ground waters; hydroxylated metabolites like Dicamba-5-hydroxy can occur during transformation. Monitoring this metabolite supports evaluation of degradation pathways, leaching potential, and persistence under varied pH and microbial activity.
Effects on Wildlife
- Primary concern relates to phytotoxicity in non-target broadleaf vegetation.
- Aquatic primary producers may be sensitive; therefore, water monitoring for parent and metabolites is recommended near treated areas.
Safety Measures
- Handle reference materials with gloves, lab coat, and eye protection.
- Prepare standards using calibrated glassware; avoid alkaline conditions that may alter analyte speciation.
- Store solutions in amber vials to protect from light; minimize freeze–thaw cycles.
Analytical Approach
- Preferred: LC-MS/MS in negative ESI with multiple reaction monitoring (MRM). Evaluate two transitions for identification where required.
- Ionization and chromatographic conditions: aqueous mobile phases with volatile buffers (e.g., ammonium fluoride, ammonium acetate) to enhance sensitivity; reversed-phase columns with endcapping suitable for acidic analytes.
- Quantification: external calibration with matrix-matched standards; where available, use stable isotope-labelled internal standards for isotope dilution to improve accuracy.
Method Validation
- Validation parameters: linearity across calibration range, LOD/LOQ compliant with target MRLs, recovery (70–120%) and precision (RSD ≤ 20%) across matrices, selectivity, and measurement uncertainty estimation.
- Conduct robustness checks for pH, extraction salt composition, and sorbent selection (WAX vs. mixed-mode).
Storage & Stability
- Solid reference materials: store at 2–8 °C or per product label, desiccated and protected from light.
- Solution standards: store at ≤ −20 °C in amber glass; verify stability before prolonged use and recheck concentration periodically.
- Avoid exposure to high pH and prolonged elevated temperatures.
Quality & Compliance
HPC Standards GmbH provides traceable reference materials manufactured and tested according to international quality requirements, supporting ISO/IEC 17025 and 17034 compliant workflows. Each lot is accompanied by a comprehensive certificate with purity, identity confirmation, and uncertainty data to facilitate defensible results.
Analytical Standards from HPC Standards
- Dicamba-5-hydroxy reference materials in neat form and ready-to-use solutions at common calibration levels.
- Optional stable isotope-labelled derivatives (subject to availability) to enable isotope dilution LC-MS/MS.
- Custom concentrations, solvent systems, and multi-analyte mixes on request for specific method scopes.
Applications
- Food and feed residue analysis aligned with regional MRLs.
- Environmental monitoring of soil, surface water, and groundwater near treated fields.
- Plant metabolism, drift, and dissipation studies supporting stewardship and regulatory submissions.
Ordering & Support
Contact HPC Standards GmbH for technical documentation, lot-specific certificates, and expert guidance on method development, matrix effects, and validation strategies for Dicamba-5-hydroxy. Customized reference materials and bulk supply are available for routine high-throughput laboratories.