Aflatoxin G2 — Reference materials for accurate residue analysis
Aflatoxin G2 — High-Purity Reference Materials for Confident Compliance
Ensure precise, defensible results with HPC Standards’ premium Aflatoxin G2 reference materials. Trusted by food and environmental laboratories, our AFG2 solutions and neat materials deliver robust calibration, method validation, and routine QC for LC-FLD and LC-MS/MS workflows. Each batch is supplied with a comprehensive CoA, metrological traceability, and concentration ranges aligned to EU, FDA, and Codex requirements—helping your team meet limits reliably across nuts, maize, spices, dried fruits, and cereals. Choose HPC Standards for consistent performance, ISO/IEC 17025-ready QA/QC support, and custom concentrations or multi-analyte mixes to streamline your calibration.
Product | Catalog No./ CAS No. | Quantity | Price | |
|---|---|---|---|---|
ISO 17034 Certified Reference Material Aflatoxin G2 solution |  | 685171 | 1X1ML | Please log in. |
HPC Standards GmbH supplies high-purity Aflatoxin G2 reference materials to support precise quantification, method validation, and routine quality control in food and environmental testing laboratories, ensuring compliance with international regulatory limits.
Overview
Aflatoxin G2 (AFG2) is a naturally occurring mycotoxin of the G-group produced primarily by Aspergillus flavus and Aspergillus parasiticus. It co-occurs with Aflatoxins B1, B2, and G1 in susceptible commodities. Under UV light (365 nm), G-group aflatoxins typically exhibit green fluorescence. AFG2 is structurally related to AFG1 (its dihydro analog) and is less potent than AFB1 but remains toxic and relevant for regulatory monitoring.
CAS: 7241-98-7 | Typical matrices: groundnuts (peanuts), maize (corn), tree nuts (e.g., pistachio, almond, hazelnut), spices, dried fruits, cereals, and derived products.
Uses
- Calibration and system suitability in LC-FLD and LC-MS/MS determination of aflatoxins in foods, feeds, and environmental samples.
- Method validation (linearity, accuracy, precision, LOD/LOQ) and recovery studies using spiking solutions.
- Ongoing quality control: control charts, proficiency testing support, and instrument performance checks.
Regulatory landscape
- European Union: Maximum levels apply to Aflatoxin B1 and the sum of AFB1, AFB2, AFG1, and AFG2 in specified commodities (e.g., peanuts, nuts, maize, and spices). Current limits are commodity-specific; laboratories should consult the latest EU regulations for exact values.
- United States (FDA): Action levels commonly reference the total aflatoxins (B1 + B2 + G1 + G2), e.g., 20 ppb in most human foods, with higher allowances in certain animal feeds depending on species.
- Global: Codex and national frameworks set comparable limits and require validated methods and traceable measurement results.
Monitoring and compliance
- Risk-based sampling plans are essential due to heterogeneous distribution; ensure adequate lot sampling and thorough comminution.
- Sample clean-up often uses immunoaffinity columns or SPE prior to chromatographic analysis.
- Regular use of independent reference materials underpins traceability, method robustness, and defensible compliance decisions.
Health impact
Human toxicity
- Acute exposure: gastrointestinal distress, hepatotoxicity, and—in severe cases—acute aflatoxicosis with liver failure.
- Chronic exposure: increased risk of hepatocellular carcinoma; risk is exacerbated by co-exposure to hepatitis B virus. AFG2 is generally less potent than AFB1 but contributes to total aflatoxin burden.
- Additional effects: growth impairment, immunosuppression, and potential micronutrient interactions with long-term dietary intake.
Environmental impact
Effects on wildlife and livestock
- Contaminated feed can reduce productivity, growth rates, and immune status in livestock; metabolite carry-over (e.g., AFM1 in milk) is a downstream concern.
- Post-harvest mold growth is favored by warm, humid conditions and poor storage; integrated management reduces occurrence.
Safety measures
- Handle Aflatoxin G2 as a potent toxic substance in a certified fume hood with appropriate PPE (lab coat, nitrile gloves, eye protection); avoid inhalation and skin contact.
- Prevent cross-contamination: dedicate glassware and surfaces, implement decontamination procedures (e.g., sodium hypochlorite for surfaces), and segregate waste.
- Dispose of solutions, consumables, and contaminated materials as hazardous chemical waste according to institutional and legal requirements.
Chemical and stability information
- Light sensitive; protect from UV/visible light using amber containers and minimal exposure during handling.
- Store neat materials and solutions refrigerated or frozen as indicated on the certificate of analysis (often ≤ -20 °C); allow solutions to equilibrate before opening to avoid condensation.
- Use suitable solvents (e.g., acetonitrile or methanol) and check solution stability over time; prepare fresh dilutions as needed for low-level analysis.
Analytical methods
- LC-FLD with post-column derivatization or photochemical activation is widely used for routine quantification of AFG2 alongside other aflatoxins.
- LC-MS/MS provides enhanced selectivity and sensitivity at low µg/kg to ng/g levels with multi-mycotoxin capability.
- Method performance characteristics should be established via recovery experiments, matrix-matched calibration, and routine use of independent reference materials.
Quality assurance (QA/QC)
- Employ matrix blanks, spikes, duplicates, and ongoing calibration verification to control bias and precision.
- Leverage reference materials for daily calibration and periodic verification to maintain traceability and support ISO/IEC 17025-compliant workflows.
- Participate in proficiency testing and apply control charts to detect drift or systematic error.
Sample preparation and matrices
- Typical workflows: homogenization, extraction with acetonitrile/water or methanol/water, clean-up (immunoaffinity or SPE), and chromatographic analysis.
- Matrix effects are significant; use matrix-matched calibration and evaluate recovery at multiple fortification levels, especially near regulatory limits.
Risk management and mitigation
- Pre-harvest: resistant varieties, biocontrol with atoxigenic Aspergillus strains, and good agricultural practices.
- Post-harvest: rapid drying, moisture control, aerated storage, sorting, and removal of damaged kernels; implement HACCP-based controls along the supply chain.
Documentation and traceability
- Each reference material batch is supplied with a certificate of analysis detailing concentration, purity profile, uncertainty, storage, and handling instructions.
- Gravimetric preparation and verification support metrological traceability for defensible reporting.
HPC Standards reference materials for Aflatoxin G2
- Formats: neat material and ready-to-use solutions; individual AFG2 or multi-analyte mixes with other aflatoxins for streamlined calibration.
- Concentration ranges tailored for regulatory monitoring in food and feed; custom concentrations and solvent systems available on request.
- Products are tested according to international quality requirements and meet the highest industrial standards, supporting reliable compliance testing.
Storage, packaging, and shipping
- Supplied in amber glass vials with tamper-evident closures to minimize photodegradation and adsorption.
- Shipped with temperature control as required; store immediately under the conditions stated on the certificate of analysis.
Applications and sectors
- Food and feed testing laboratories, grain handlers, nut processors, spice traders, contract research organizations, and regulatory authorities.
- Supports screening, confirmatory analysis, and release testing in QA laboratories and compliance programs.
Related reference materials
- Aflatoxin B1, B2, G1, AFM1, and multi-mycotoxin solutions for comprehensive surveillance.
- Stable isotope-labelled derivatives may be available for advanced isotope dilution workflows; inquire for current availability.
Notes on regulations
- Limits and method requirements are periodically updated. Always verify current national and international regulations and adopt validated methods aligned with recognized standards (e.g., AOAC/ISO/CEN).